It was shown that the transcription initiation of the kanamycin resistance gene (the Km gene) from transposon Tn5 in E.coli HB101 strain can be provided by specific yeast sequences. To localize a region of the yeast DNA involved in transcription initiation, a number of hybrid plasmids ...
The evolved kanamycin-resistance gene products could be used as selection markers at the optimum growth temperature of T. thermophilus* The development of such a convenient genetic-engineering tool would facilitate post-sequencing research on T. thermophilusJournal ...
pEGFP-C1是穿梭载体，neomycin/kanamycin resistance gene指在大肠杆菌中是卡那霉素抗性，在另外一个宿主中...
Protein fusions with the kanamycin resistance gene from transposon Tn5. The gene for the neomycin phosphotransferase II (NPT II) from transposon Tn5 was fused at the amino or carboxy terminus to foreign DNA sequences coding for... Reiss,Sprengel,Schaller - 《Embo Journal》 被引量: 199发表: ...
1.1Antibiotic resistance The ABCG transporter subfamily member ABCG19 has been reported to provide kanamycin resistance to plants upon overexpression (Mentewab and Stewart 2005). Mutants ofA. thalianawhere the AtWBC19 or ABCG19 gene was knocked-out showed much slower root growth in presence of ...
In this project we have analysed the use of an intron-containing neomycin phosphotransferaseII – nptII – gene. The advantage of this construct is that only eukaryotic organisms will be able to process this gene. Accordingly, the theoretical risk of horizontal gene flow of antibiotic resistance g...
Azide Agar and for selection of transformed plant cells containing the neomycin phosphotransferase on a kanamycin-medium. Kanamycin sulfate can also be used as a selection agent for cells transformed with kanamycin B resistance gene. It is recommended for use in cell culture applications at 100 mg/...
The other IS50 element contains a mutant allele of the transposase/inhibitor gene, inactivated by a point mutation that created the promoter for expression of the NPTII kanamycin resistance gene in Tn5's unique central region (loop in Figures 1a and b). The Journal of Antibiotics Julian Davies...
The group B isolates appeared to show a previously undocumented increased resistance to the aminoglycosides gentamicin and kanamycin. A prospective study of ... Reid,T M - 《British Medical Journal》 被引量: 103发表: 1975年 Activity of gentamicin against Pseudomonas and hospital Staphylococci. No ...
It can be used as a selective agent in cell culture media to inhibit the growth of cells that do not contain a specific resistance gene. Additionally, it can be used as a tool in molecular biology experiments to study gene expression and protein synthesis. Storage Conditions: Store in a...