K562 cells can be further modified to express CD19 together with membrane-bound cytokines, such as IL-15, for propagating clinical-grade CD19-specific CAR T cells [13,68]. More recently, K562 cells engineered to express a membrane-bound form of anti-CD3 monoclonal antibody (mAb) (mOKT3) ...
Flow cytometry and chromosomal analysis of bone marrow cells indicated positive findings of CD10, CD19, CD34, HLA-DR antigens and t(9; 22)(q34; q11.2), respectively. No rearrangements of bcr/abl in peripheral blood neutrophils were found by fluorescence in situ hybridization, suggesting that...
Flow cytometry with UV excitation light was used to detect the percentage of SP cells in logarithmic growth periodK562, which were then sorted by the fluorescence activating cell sorter, and then SP and non-SP subpopulations were collected.The expression of multidrug resistance proteins were examined...
An appropriate assay system monitors the expression of the early activation marker CD69 in whole blood after stimulation with extracts. CD69 is expressed in all activated lymphocyte subsets and hence it represents a generic marker to monitor individual subset responses to specific stimuli [33]. CD4...
According to the invention, the method comprises the following steps: transcribing expression carriers, which are used for stably expressing CD8 alpha-interleukin 21, CD14, CD19, CD86 and CD137, by using K562 cell lines, wherein the CD8 alpha is a membrane protein expressed on a cell membrane...
These clones retained natural cytotoxicity, but most of them had lower CD16 and HLA-DR expression levels, especially when compared to clones cultivated according to model 1. Above all, we demonstrated that cryopreservation of well-proliferating NK cell clones cultivated using model 2 had almost no...