ThT stock solution was prepared in double distilled water and filtered with 0.2 micron millipore filter. HEWL samples incubated with or without vitamin k3, from each set samples were withdrawn at definite interval of time and mixed with ThT to accomplish final protein and dye concentration of 15...
(B) The number of LMTK3-KCC2 colocalized clusters per 100 micron of dendrite was then determined; n = 17 neurons from 3 cultures. (C) Brain sections were subject to immunostaining and confocal microscopy as outlined earlier. (D) The percentage of LMTK3 puncta that contain KCC2 was then...
ThT stock solution was prepared in double distilled water and filtered with 0.2 micron millipore filter. HEWL samples incubated with or without vitamin k3, from each set sam- ples were withdrawn at definite interval of time and mixed with ThT to accomplish final protein and dye concen- tration...