k252a~够抑制BDNF—TrkB 信号传导通路对Arc表达的诱导作用从而使癫痫发作的持续时间缩短。 【关键词】 杏仁核;癫痫;活性调节细胞骨架蛋白;k252a;脑源性神经生长因子 k252ainhibits BDNF -- TrkBsignalingpathwaytoefecttheexpressionofArcinelectrical stimulationkindledratsandepilepsy ...
性状：灰白色至浅黄色固体粉末 储藏条件 ：-20°C 3 年 ；4°C 2 年 / 溶液中:-80°C 6 月； -20°C 1月 使用案例 图2.TrkB受体抑制剂K252a对神经突生长的影响。原代海马神经元在存在或不存在200 nM K252a的情况下用10 nM PACAP或2 nM BDNF培养3 DIV，并对pNF和MAP2进行双重免疫染色。显示了神经...
癫痫活性调节细胞骨架蛋白k252a脑源性神经生长因子目的 探讨BDNF - TrkB信号传导通路与杏仁核电刺激点燃大鼠Arc表达及癫痫发生的关系.方法 雄性SD大鼠随机分为空白组,假手术组,杏仁核点燃组(单纯点燃组,k252a注射组,DMSO对照组).应用Alpha - Lab 4通道信号采集及处理系统记录大鼠脑电活动.运用免疫组化检测其Arc蛋白的...
在人胃癌细胞和猪卵母细胞中发现BDNF作用于原肌球蛋白相关激酶B型(Tropomyosin-related kinase B,TrkB)受体促进靶细胞中PTX3生成。本研究拟通过BDNF干预脑I/R大鼠,... 贺显淑 - 川北医学院 被引量: 0发表: 2019年 脑源性神经营养因子通过TrkB-MAPK-Erk1/2途径保护丙烯酰胺诱导的神经元和突触损伤 目的:建立丙...
BDNF对食管癌细胞株ECa9706体外侵袭能力的影响 目的:本研究观察脑源性神经营养因子(brain-derived neurotrophic factor,BDNF)对食管癌细胞株ECa9706体外增殖和迁移能力的影响以及机制.方法:采用MTT和Transwell方法... 冯睿婷,梅家转,栗敏,....
Our results demonstrated that K252a, was able to attenuate BDNF-induced migration and proliferation of NPC cells. More importantly, we demonstrated for the first time that K252a harbored potent anoikis-sensitization activity (i.e. sensitizing cancer cells to detachment-induced cell death) against EBV...
Guo D,Hou X,Zhang H,et al.More expressions of BDNF and TrkB in multiple hepatocellular carcinoma and anti-BDNF or K252a induced apoptosis,supressed invasion of HepG2 and HCCLM3 cells. Journal of Experimental and Clinical Cancer Research . 2011...
Interestingly, we previously demonstrated that k252a reversed H2S alleviated Hcy-induced endoplasmic reticulum stress and neuronal apoptosis via inhibiting BDNF-TrkB pathway. Therefore, we assumed that k252a might inhibit the protective effect of H2S on Hcy-induced cognitive dysfunction. We used Hcy to ...
Western blotting观察大鼠海马BDNF,CREB,ERK1/2,BCL-2蛋白表达的变化.结果 与空白对照组比较,CUMS组大鼠的活动量,糖水消耗量,学习记忆能力显著降低(P<0.05或P<0.01),HPA轴功能亢进(P<0.01),血清单胺递质水平降低(P<0.01),BDNF,CREB,ERK1/2,BCL-2的表达下调(P<0.01或P < 0.05),而DMSO组上述指标差异无...
BDNF release and effect of exogenous BDNF, pro-BDNF, TrkB inhibitor (K252a) and neutralizing anti-BDNF on apoptosis and proliferation in metastatic CRC cell lines.Hussein, Akil