000× and object pixel size of 1.16 Å. Movies were dose-fractionated into 200 ms frames for atotal exposure of 12 s with a dose rate of ~5.1 electrons Å−2 −s−1. For the LRRK1(FL) sample, multiple datasets were collected and combined as the addition of ...
Complementary DNA was generated using equal amounts of RNA from each sample and the iScript complementary DNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions. Reactions were carried out using SsoFast probes mixture (Bio-Rad) and TaqMan primer probes (Thermo Fisher Scientific)...
This bacterial mixture was mixed with the filtered wastewater sample and incubated at 37 °C with shaking for 72 h. Samples were collected every 24 h and centrifuged, and overlay agar assays were performed to identify phage-sensitive bacterial strains, as previously described [53]. The enrichment...
The determination of the antibody titer, anti-LANA, of each sample was evaluated by serial dilutions of the plasma (1:40, 1:80, 1:160). The positivity was defined by the presence of dotted nuclear reactivity at each dilution. The results of each dilution were interpreted by three ...
The determination of the antibody titer, anti-LANA, of each sample was evaluated by serial dilutions of the plasma (1:40, 1:80, 1:160). The positivity was defined by the presence of dotted nuclear reactivity at each dilution. The results of each dilution were interpreted by three ...