For both samples, 4 μl of sample was applied to a freshly plasma-cleaned Ultrafoil grid (Electron Microscopy Sciences). Samples were blotted for 4 s using a blot force of 20 followed by vitrification using a Vitrobot Mark IV (FEI) set to 4 °C and 100% humidity. Data ...
Complementary DNA was generated using equal amounts of RNA from each sample and the iScript complementary DNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions. Reactions were carried out using SsoFast probes mixture (Bio-Rad) and TaqMan primer probes (Thermo Fisher Scientific)...
The determination of the antibody titer, anti-LANA, of each sample was evaluated by serial dilutions of the plasma (1:40, 1:80, 1:160). The positivity was defined by the presence of dotted nuclear reactivity at each dilution. The results of each dilution were interpreted by three ...