The proliferation index was determined by Ki-67 immunostaining and calculating the ratio of Ki-67-positive cells among total number of cells in five randomly selected fields at × 200. The sections were stained with anti-mouse CD105 monoclonal antibody (BD Pharmingen, San Diego, CA, USA). ...
To evaluate the proliferative index of tumour tissues, the Ki-67 labelling index was determined following the method described by Shaffrey et al (2005). Briefly, five views of each slide were randomly selected at high-power magnification ( Â 40), among which the percentage of Ki-67 cells...
IHC assays of mouse xenograft tumor tissues were performed by Servicebio (Wuhan, China). Proliferative markers were detected using anti-Ki-67 (Bioss, Beijing, China). Images were obtained using a fluorescence microscopy (Lecai, Germany). RNA fluorescence in situ hybridization (FISH) assay FISH as...
Ki-67 labelling index and invasiveness among anterior pituitary adenomas: analysis of 103 cases using the MIB-1 monoclonal antibody. AIMS: To investigate the relation between proliferative activity of anterior pituitary adenomas, quantified by the Ki-67 labelling index, and their invasiv... L Mastron...
However, the siRNA-mediated repression of FHL2 in FOXK1-overexpressing cells reversed EMT and both the proliferative and metastatic phenotypes in vitro and in vivo. These data identified that the co-expression of FOXK1 and FHL2 enhances cell proliferation and metastasis through the induction of ...
(Prom1)-derived proliferative tumor-propagating HCC cells in vivo8. These cells display a trajectory of dedifferentiation towards more embryonic-like and epithelial mesenchymal transition (EMT) features; while targeted depletion of the CD133/Prom1-lineage resulted in the reviving of hepatic functional ...
et al. A spontaneous mutation characterized by chronic proliferative dermatitis in C57BL mice. Am. J. Pathol. 143, 972–982 (1993). CAS PubMed PubMed Central Google Scholar Gerlach, B. et al. Linear ubiquitination prevents inflammation and regulates immune signalling. Nature 471, 591–596 (...
(Supplementary Fig.4b), accompanied by enhanced migration and invasion (Supplementary Fig.4c–d). However, these effects were almost completely eliminated by SPINK1-specific shRNAs (Supplementary Fig.4a), which retained normal proliferative potential of stromal cells but resulted in essentially reversed...