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Like other PB1 domains, the MEKK3PB1 adopts a ubiquitin-like fold consisting of a five-stranded b-sheet, an a-helix inserted between strands b2 and b3, and a second helix between b4 and b5 (ref. 14). MEKK3 grasps the CCM2HHD, with the thumb (MEKK3N) resting in the groove ...
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CD analysis suggested that these three proteins possess some beta-sheet structure, with little alpha-helix except for proteinase R which showed about 14% alpha-helix. SDS-PAGE and gel filtration in 0.1% SDS indicated that proteinase T and K are resistant to SDS-induced unfolding similar to ...
21 View Batch SDS Reconstitution Calculator Molarity Calculator Reconstitution Calculator The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest...
Product Datasheets COA view current batch SDS Reconstitution Calculator Molarity Calculator Reconstitution Calculator The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculato...
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The methylation activity of purified WT PfSET and mutant PfSET proteins was carried out with synthetic peptides (region spanning H3K9, H3K27, H3K36, H3K64, H3K64A) (Supplementary File 2, sheet 6). The reaction mixture was prepared in the methylation buffer (250-mM, Tris pH 9, 20-mM...
Construction of K-Pop Dance Database A K-pop dance database was constructed containing 200 point-dance movements from four proAfeKss-iponoapl ddaanncceerdsa(ttawboasemwenasacnodnstwtruoctwedomceonn)taoinbitnaigne2d00bpyoaintm-doatinocne mcaopvtuerme esnytsstefmromthafot ur propfreosdsuiocne...
Sonication of lysed nuclei (lysed in a buffer containing 1% SDS) was performed on a BioRuptor UCD-300 for 60 cycles, 10 s on 20 s off, centrifuged every 15 cycles, chilled by 4°C water cooler. Samples were checked for sonication efficiency using the criteria of 150-500 bp by gel ...