A high-pressure liquid chromatography with electrospray ionization triple quadrupole mass spectrometer (HPLC/MS/MS) method was validated for the analysis of JWH-018 in the specimens using deuterated Δ(9) -tetrahydrocannabinol (d(3) -THC) as the internal standard. JWH-018 was isolated by cold ...
Liquid Chromatography-Tandem Mass Spectrometry Analysis of Urine Specimens for K2 (JWH-018) Metabolites Marijuana is the most widely used drug of abuse all over the world. The major active constituent of the drug is 螖9-tetrahydrocannabinol (螖9-THC). 螖9-THC... ElSohly Mahmoud A.,G Waseem...
Conjugates were identified and characterized using liquid chromatography/tandem mass spectrometry, whereas kinetic parameters were quantified using high-performance liquid chromatography-UV-visible methods. UGT1A1, UGT1A3, UGT1A9, UGT1A10, and UGT2B7 were shown to be the major enzymes involved, ...
. Liquid–liquid extraction (LLE) was performed twice with 1ml hexane/ethyl acetate 99+1 (v/v) by gentle shaking for 15min. After centrifu- gation and evaporation of the organic layers, the residue was reconstituted in 150 l HPLC eluent and 25 l was injected. 2.5. Method validation The...
[4]Teske J;Weller JP;Fieguth A .Sensitive and rapid quantification of the cannabinoid receptor agonist naphthalen-1-yl-(1-pentylindol-3-yl) methanone (JWH-018) in human serum by liquid chromatography-tandem mass spectrometry[J]. Journal of Chromatography B, 2010,27. ...
Khan, Liquid chromatography-tandem mass spectrometry analysis of urine specimens for K2 (JWH-018) metabolites, J. Anal. Toxicol. 35 (2011) 487-495, http://dx.doi.org/ 10.1093/anatox/35.7.487.ElSohly MA, Gul W, ElSohly KM, et al. (2011). Liquid chromatography- tandem mass ...
Also, liquid-liquid extraction at pH 10.2 with ethyl ether was conducted and the tandem mass spectrometry was performed with positive electroscopy ionization mode.Kevin G ShanksAIT LaboratoriesIndianapolisTim DahnAIT LaboratoriesIndianapolisAndrea R Terrell...
Methods High-performance liquid chromatography (HPLC) coupled with triple quadrupole mass spectrometry (QqQ-MS) and linear ion trap time-of-flight mass spectrometry (IT-TOF-MS) using electrospray ionization (ESI) in its positive ion mode were utilized to analyze six model compounds, FUB-JWH-018 ...
JWH-018 was isolated by cold acetonitrile liquid-liquid extraction. Chromatographic separation was performed on a Zorbaz eclipse XDB-C 18 column. The assay was linear from 1 to 1000ng/mL. Six C57BL6 mice were sacrificed 20min after exposure to the smoke of 200mg 'Buzz' containing 5.4% JWH...
liquid chromatography–mass spectrometryEach year, synthetic cannabinoids occur in high numbers on the illicit drug market, but data on their detectability are rarely available. A pilot study was performed to assess adverse effects of JWH-018, which is one of the oldest and best known synthetic ...