mRNA的表达的下降,Jurkat细胞表面分子CD4/CD8的表达无显著改 变,证明Jurkat细胞中CD4/CD8的表达与ZBTB7B的表达不相关。 关键词:锌指结构转录调节因子(ZBTB7B);P14ARF;重组腺病毒; 转染 重庆医科大学硕士研究生学位论文 EFFECTOFSIRNATARGETINGZBTB7BONTHE EXPRESSIONOFCD4/CD8ANDP14AI江INJURKAT CELLS ABSTRACT Objective...
Nearly all human CD4+ T cells and 50% of human CD8+ T cells express CD28. The CD28 interaction with CD80 (aka B7-1) or CD86 (aka B7-2) on APCs, in conjunction with TCR engagement, triggers a co-stimulation signal (signal 2). It results in T cell proliferation, cytokine production...
Nearly all human CD4+ T cells and 50% of human CD8+ T cells express CD28. The CD28 interaction with CD80 (aka B7-1) or CD86 (aka B7-2) on APCs, in conjunction with TCR engagement, triggers a co-stimulation signal (signal 2). It results in T cell proliferation, cytokine production...
Detection of GABA-A channel subunit proteins in CD4+ and CD8+ T cells from rats, mice and the Jurkat cell line.Suresh, K. MenduAmol, BhandageZhe, JinBryndis, Birnir
We and others have shown that adoptive immunotherapy using A/B TCR expressing cytotoxic T cells will augment the eradication of tumor in a SCID model (11, 12). The capability of CTLs to cause tumor regression is thought to be far greater than a humoral response or a CD4 -mediated Th ...
在人类肿瘤和老鼠模型中,Tal1高表达与胸腺细胞CD4+CD8+阶段分化阻滞相关,而Jurkat细胞是CD4+CD8+T-ALL细胞,而且TAL1 异常表达,在多种T-ALL分子研究中被应用[2, 5],所以在我们的研究中应用了Jurkat 细胞作为研究对象。Jurkat 细胞属于WT型Tal1高表达的细胞株,为了更确切的说明Tal1对T-ALL细胞增殖的影响,下一...
Jurkat 细胞免疫表型:CD2+、CD3+、CD4+、CD6+、CD7+、CD8-、CD19-、CD34+、TCRα/β+、 TCRγ/δ-。Jurkat 细胞是亚四倍体,细胞遗传学特点:87(78-91) 4nXX, -Y, -Y, -5, -16, -17, -22, (2)(p21)/del(2)(p23)x2, der(5)t(5;10)(q11;p15), del(9)(p11)。针对上述免疫学和...
DC诱导的T细胞表型为实验组,未经DC诱导的T细胞表型为对照组,收集培养的细胞悬液,洗涤、离心后加PBS悬浮,加入CD3、CD4、CD8及CD28荧光抗体,用流式细胞仪检测。 细胞毒作用的乳酸脱氢酶(LDH)释放法测定 收集上述方法培养的活化T细胞作为效应细胞,取传代后24小时的Jurkat细胞为靶细胞,效靶比为5∶1、10∶1、20∶1。
1B) which was confirmed by the significant decrease of number of trypan blue-negative cells (Fig. 1C). The cell number reduction could be due to oxLDL inhibitory effect on cell proliferation and/or to a cell death increase. In the presence of 200 μg apoB/ml oxLDLs, DNA synthesis (...
CD28 is a homodimeric and transmembrane protein expressed by T cells. Nearly all human CD4+ T cells and 50% of human CD8+ T cells express CD28. The CD28 interaction with CD80 (aka B7-1) or CD86 (aka B7-2) on APCs, in conjunction with TCR engagement, triggers a co-stimulation sign...