Nonhomologous end joining (NHEJ) is the primary pathway of DNA double-strand-break repair in vertebrate cells, yet how NHEJ factors assemble a synaptic complex that bridges DNA ends remains unclear. To address the role of XRCC4-like factor (XLF) in synap
Double-strand break A break in both strands of a duplex DNA molecule. End joining Generic term for ligation of the ends of a double-strand break. Endogenous Originating from within. An enzyme that cuts DNA between two bases. Decompacted chromatin that often contains actively transcribed genes. ...
P. Menage a trois: double strand break repair, V(D)J recombination and DNA-PK. Bioessays 17, 949–957 (1995). 6. Hammel, M. et al. Ku and DNA-dependent protein kinase dynamic conformations and assembly regulate DNA binding and the initial non-homologous end joining complex. J. Biol....
We suggest a model where Ku utilizes an unusual characteristic of its three-dimensional structure to recognize certain protein-occluded ends without the extensive remodeling of chromatin structure required by other DNA repair pathways. The nonhomologous end joining pathway for DNA double strand break ...
The slatted base consists of a side (1) with a fold stuck to it by a padded strip (4). The sprung slats (5) are stuck to the base surfaces by a hooked-pile fastener strip or hooked strip (6), and rest on the padded strip. At the two ends, the end piece (3) and side-...
Cultured cells from FA patients display a high level of spontaneous chromosome breaks and an increased frequency of intragenic deletions, suggesting that FA cells may have deficiencies in properly processing DNA double strand breaks. In this study, an in vitro plasmid DNA end joining assay was used...
opening the hairpin coding ends. Biochemical studies have shown that the Mre11 protein, which is involved in double-strand break repair, can efficiently open hair- § To whom correspondence should be addressed (e-mail: davidbr@bcm.tmc.edu). pins in the presence of its partner protein, Nbs1...
Humans have evolved a series of DNA double-strand break (DSB) repair pathways to efficiently and accurately rejoin nascently formed pairs of double-stranded DNA ends (DSEs). In G0/G1-phase cells, non-homologous end joining (NHEJ) and alternative end joining (A-EJ) operate to support covalen...
Ionizing radiation (IR) induces DNA double-strand breaks (DSBs), which can be repaired via two main pathways: Non-homologous end-joining (NHEJ) and homologous recombination (HR). NHEJ involves recruitment of DNA-dependent protein kinase catalytic subunit (DNA-PKcs) to DNA ends by the Ku70/80...
such as replication of DNA, separation of chromosomes, and transcription of genes. An intact DNA is a prerequisite for the integrity of genetic information and, therefore, should be maintained at all costs. Errors such as double strand breakage are so crucial that they can result in cell death...