The slatted base consists of a side (1) with a fold stuck to it by a padded strip (4). The sprung slats (5) are stuck to the base surfaces by a hooked-pile fastener strip or hooked strip (6), and rest on the padded strip. At the two ends, the end piece (3) and side-...
S. SFPQ*NONO and XLF function separately and together to promote DNA double-strand break repair via canonical nonhomologous end joining. Nucleic Acids Res. 45, 1848–1859 (2017). CAS PubMed Google Scholar Li, S. et al. Cell-type specific role of the RNA-binding protein, NONO, in the...
NHEJ of incompatible 3′ ends was not improved with XLF or PAXX (Fig. 7B, lanes 2–5). The same was observed for the NHEJ of DNA with a 3′ overhang with a blunt-ended DNA partner (Fig. 7B, lanes 7–10). However, NHEJ of blunt-ended DNA was stimulated ∼2-fold by PAXX bu...
Double-strand breaks NHEJ Stages Scaffolds Strings X-ray diffraction Cryo-EM Abbreviations 53BP1 p53-binding protein 1 APLF aprataxin and PNKP-like factor BRCT BRCA1 C-Terminus domain cryo-EM cryo-electron microscopy CTR C-terminal region DNA-PK DNA-dependent protein kinase DNA-PKcs DNA-PK catal...
Abbreviations DSB: DNA double-strand break; CSR: Class-switch recombination; HR: Homolo- gous recombination; NHEJ: Non-homologous end-joining; C-NHEJ: Classical non-homologous end-joining; A-NHEJ or A-EJ: Alternative non-homologous end-joining pathways; AT: Ataxia-telangiectasia; DNA-PK: DNA...
DNA double-strand breaks (DSBs) are perhaps the most deleterious threat to genomic stability. Cells use two main pathways to repair DSBs: non-homologous end joining (NHEJ) and homologous recombination (HR)2. These two pathways are largely distinct from one another. HR is particularly effective ...
opening the hairpin coding ends. Biochemical studies have shown that the Mre11 protein, which is involved in double-strand break repair, can efficiently open hair- § To whom correspondence should be addressed (e-mail: davidbr@bcm.tmc.edu). pins in the presence of its partner protein, Nbs1...
Fig. S9 shows that addition of a 5- to 20-fold molar excess of N added in trans reduces the yield of HJ with the ΔN mutant but not with the hyperactivated ΔN; D202A double mutant. Download: Download high-res image (1MB) Download: Download full-size image Figure 7. Recombination ...
Double-strand break A break in both strands of a duplex DNA molecule. End joining Generic term for ligation of the ends of a double-strand break. Endogenous Originating from within. An enzyme that cuts DNA between two bases. Decompacted chromatin that often contains actively transcribed genes. ...
Such a molecule may also be prepared by linearization of a circular double strand DNA molecule by restriction enzyme digestion, with subsequent attachment of synthetic oligomer linkers onto the ends, or secondary digestion of an already linear DNA molecule with restriction enzymes, followed by addition...