The slatted base consists of a side (1) with a fold stuck to it by a padded strip (4). The sprung slats (5) are stuck to the base surfaces by a hooked-pile fastener strip or hooked strip (6), and rest on the padded strip. At the two ends, the end piece (3) and side-...
S. SFPQ*NONO and XLF function separately and together to promote DNA double-strand break repair via canonical nonhomologous end joining. Nucleic Acids Res. 45, 1848–1859 (2017). CAS PubMed Google Scholar Li, S. et al. Cell-type specific role of the RNA-binding protein, NONO, in the...
Double-strand break A break in both strands of a duplex DNA molecule. End joining Generic term for ligation of the ends of a double-strand break. Endogenous Originating from within. An enzyme that cuts DNA between two bases. Decompacted chromatin that often contains actively transcribed genes. ...
NHEJ of incompatible 3′ ends was not improved with XLF or PAXX (Fig. 7B, lanes 2–5). The same was observed for the NHEJ of DNA with a 3′ overhang with a blunt-ended DNA partner (Fig. 7B, lanes 7–10). However, NHEJ of blunt-ended DNA was stimulated ∼2-fold by PAXX bu...
DNA double-strand breaks (DSBs) leading to loss of nucleotides in the transcribed region can be lethal. Classical non-homologous end-joining (C-NHEJ) is the dominant pathway for DSB repair (DSBR) in adult mammalian cells. Here we report that during such DSBR, mammalian C-NHEJ proteins form...
Abbreviations DSB: DNA double-strand break; CSR: Class-switch recombination; HR: Homolo- gous recombination; NHEJ: Non-homologous end-joining; C-NHEJ: Classical non-homologous end-joining; A-NHEJ or A-EJ: Alternative non-homologous end-joining pathways; AT: Ataxia-telangiectasia; DNA-PK: DNA...
At E9.5, double mutant embryos show growth delay in head, heart, and somites. At E10.5, most Ku80 Dido double mutant embryos are being resorbed. Magnification, 40-fold. All animal experiments were per- formed in compliance with EU and CNB animal committee directives. Guerrero et al. Cell ...
opening the hairpin coding ends. Biochemical studies have shown that the Mre11 protein, which is involved in double-strand break repair, can efficiently open hair- § To whom correspondence should be addressed (e-mail: davidbr@bcm.tmc.edu). pins in the presence of its partner protein, Nbs1...
DNA double-strand break (DSB) repair by non-homologous end joining (NHEJ) in human cells is initiated by Ku heterodimer binding to a DSB, followed by recruitment of core NHEJ factors including DNA-dependent protein kinase catalytic subunit (DNA-PKcs), XRCC4-like factor (XLF), and XRCC4 (...
Double-strand breaks NHEJ Stages Scaffolds Strings X-ray diffraction Cryo-EM Abbreviations 53BP1 p53-binding protein 1 APLF aprataxin and PNKP-like factor BRCT BRCA1 C-Terminus domain cryo-EM cryo-electron microscopy CTR C-terminal region DNA-PK DNA-dependent protein kinase DNA-PKcs DNA-PK catal...