The technique of JC-1 staining has been developed with the intent to detectDYin intact, viable cells. For this purpose JC-1 acts as a marker of mitochondrial activity, since the formation of J-aggregates, which give red emission, is reversible. Cells with highDYare those forming J-aggregat...
Add 0.5mL JC-1 staining working solution, invert several times to mix. Incubate in a cell incubator at 37°C for 20 minutes. During the incubation period, prepare an appropriate amount of JC-1 staining buffer (1×) at the ratio of adding 4 mL of distilled water per 1 mL of JC-1 st...
JC-1 是一种广泛用于检测线粒体膜电位△Ψm的理想荧光探针。它以电势依赖性的方式积聚在线粒体内,可以用来检测细胞、组织或纯化的线粒体膜电位。正常细胞线粒体内膜电位较高,JC-1 聚集在线粒体的基质中形成聚合物,可以产生红色荧光;而在受损的线粒体中由于膜电位的下降或丧失,JC-1 不能聚集在线粒体的基质...
The dyes have low cytotoxicity, allowing stained cells to be sorted and otherwise cultured or assessed with functional assays after staining. Fixed cell assays: Analyzing cell cycle using FxCycle Violet Stain (Cat. No. F10347), SYTOX AADvanced Dead Cell Stain Kit (Cat. No. S10349) or Fx...
Measurement of mitochondria membra ne potential by JC-1 staining. HeLa cells are incubated with or without WOs for 24 h, followed by NIR irradiation for NIR and WOs+ NIR groups (JC-1 purchased from MCE).[1] 参考文献 1. Fei Wang, et al. Urchin-like tungsten suboxide forphotoacoustic ima...
Prepare the JC-1 staining solution by diluting the reagent in pre-warmed culture media. For the staining of adherent cells, JC-1 is diluted in medium to a final concentration of ~ 5 ug/mL (vortex during dilution to prevent the formation of precipitates). Mix well to dissolve all ...
JC-1 dye is a commonly used tool used for studying mitochondrial membrane potential, providing valuable insights into mitochondrial health and activity. JC-1 staining can be used as an indicator of mitochondrial membrane potential in a variet...
Foxp3/Transcription Factor Staining Buffer Set(货号00-5523)或Intracellular Fixation & Permeabilization Buffer Set(货号88-8824) Annexin V细胞凋亡检测试剂盒(货号88-8103, 88-8007, 88-8006, 88-8005, 88-8102,或88-8008) 10X结合缓冲液 ...
Measurement of mitochondria membrane potential by JC-1 staining. HeLa cells are incubated with or without WOs for 24 h, followed by NIR irradiation for NIR and WOs+ NIR groups (JC-1 purchased from MCE).[1] 精彩往期 ◆JC-1/FITC,让我们来...
流式细胞术检测JC一1单体的绿色荧光可反应线粒体膜电位的变化。 关 键 词:线粒体膜电位;碳酰氰基一对一氯苯腙;流式细胞术 中图分类号:R363 文献标识码:A EffectofCCCPonendothelialcellsmitochondrialmembranepotentialby JC-lsinglestainingdetection GUANFuxiI1,ZHOULulu。