On excitation at 490 nm, JC-1 monomers display a cytoplasmic fluorescence emission centered at 537 nm (green band).Beyond a critical concentration, JC-1 aggregates in mitochondrium, thus leading to the emergence of an intense emission band centered at 597 nm (red band), in addition to the...
JC-1单体(J-monomer)的激发波长为514nm,发射波长为529nm;JC-1聚合物(J-aggregates)的激发波长为585nm,发射波长为590nm。 JC-1可以透过细胞质膜进入细胞,以单体状态游离于胞质内,正常健康细胞的线粒体的膜电位较高,具有极性,JC-1依赖于膜电位的极性被迅速摄入线粒体的基质内,并因浓度增高而在线粒体内形成...
4. Fluorescence pattern too much widespread: see point 3.4. Do not consider any event with a very high FL2 fluorescence: very often they are JC-1 aggregates. Increase FSC threshold and discard debris with electronic gating: the presence of stained debris or broken cells can constitute a confo...
While in cells with low ΔΨm, JC-1 remains in the monomeric form. When excited at 490 nm, JC-1 monomers emit a green fluorescence with a maximum at ~520 nm. Aggregates of JC-1 emit an orange-red fluorescence with a maximum at ~590 nm. 品牌介绍:• MCE (MedChemExpress) 拥有数...
this dye accumulates and aggregates in the mitochondria, where it forms bright red fluorescent agglomerates. Any event that dissipates the mitochondrial membrane potential prevents the accumulation of the JC-1 dye in the mitochondria and thus, the dye remains in the cytoplasm in its monomer form,...
While in cells with low ΔΨm, JC-1 remains in the monomeric form. When excited at 510 nm, JC-1 monomers emit a green fluorescence with a maximum at ~527 nm. Aggregates of JC-1 emit an orange-red fluorescence with a maximum at ~590 nm. 品牌介绍:• MCE (MedChemExpress) 拥有数...
With the aim of improving the sensitivity of such analysis, we have evaluated JC-1, a fluorescent lipophilic carbocyanine dye. Above a critical concentration, JC-1 aggregates in a 'liquid crystal' form. Aggregates display a specific red emission band centered at 597 nm whereas the monomers ...
1 accumulates in mitochondria, selectively generating an orange J-aggregate emission profile (590 nm) in healthy cells. After cell injury, as membrane potential decreases, JC-1 monomers are generated, resulting in a shift to green emission (529 nm). The principal advantage of JC-1 relative to...
JC-1 forms complexes known as J-aggregates. While in cells with low ΔΨm, JC-1 remains in the monomeric form. When excited at 510 nm, JC-1 monomers emit a green fluorescence with a maximum at ~527 nm. Aggregates of JC-1 emit an orange-red fluorescence with a maximum at ~590 nm...