and screen a more simple method for clinic laboratory to detecte the mutation of JAK2V617F.[Method]DNA was extracted by standard procedures after isolating total leukocytes from peripheral blood mononuclear cells by density gradient centrifugation over Histopaque 1077...
Using a sensitive quantitative polymerase chain reaction (PCR) approach, we thought to search for JAK2V617F mutation in a cohort of normal donors, and have detected it in around 10% of healthy volunteers. Three blood samples were taken from 57 healthy donors – aged from 23 to 52 years –...
The invention relates to a TaqMan-COLD-PCR method for detecting the JAK2V617F mutation rate in peripheral blood. In the method, the DNA of a peripheral blood genome is used as a detection sample, the detection is completed within one reaction system, the JAK2V617F mutation percentage is ...
关键词:JAK2V617F突变骨髓增殖性疾病白细胞增多血小板增多红细胞增 多 111 浙江人学硕{-q:位论文 英义摘耍 To investigate JAK2V617Fmutationin patients withoneor morelinesbloodcells proliferation and analysis ofitsclinical -●～ significance Department ...
The JAK2V617F mutation is detectable at very low level in peripheral blood of healthy donors. Leukemia 2006;20(9): 1622. P Sidon,H El Housni,B Dessars,... - 《Leukemia》 被引量: 172发表: 2006年 Detection of Kaposi's sarcoma associated herpesvirus (KSHV) in peripheral blood of HIV-in...
3报道的 结果相符.此外,Kralovics等o 11J2 3观察的结果提 示:JAK2V617F基因突变阳性患者与阴性患者相 比,病程明显延长,出血,血栓,纤维化的并发症 发生率高,需用更多的细胞生长抑制剂治疗. References: [1]WOLANSKYI A P,LASHO T L,SCHWAGER S M, et a1.JAK2 617F mutation in essential thrombocy th...
结论ET患者存在较高的JAK2 V617F突变率,临床早期检测JAK2 V617F突变及突变负荷可能对ET患者血管性事件早期预防具有重要的临床价值。 关键词: 血小板增多,自发性;JAK2 V617F突变;凝血功能 ABSTRACT ObjectiveTo investigate the frequency of JAK2 V617F mutation and JAK2 V617F mutation allele burden in patients...
tive for the JAK2 V617F mutation. The table 1 shows these results. The patient with primary myelofibrosis was allo- grafted from his HLA identical sibling; interestingly, the detection of the V617F affecting the JAK2 gene has diminished substantially as the patient becomes a chimera. DISCUSSION...
JAK2 V617F Mutation Testing in Patients Presenting With Hepatic and Portal Vein Thrombosis Charles Elliott Foucar, MD1; Brady Lee Stein, MD, MHS2 Author Affiliations JAMA. 2017;317(21):2228-2229. doi:10.1001/jama.2017.2329 Full Text A 27-year-old woman with asthma and obesity recently ...
关键词 骨髓增殖性疾病 红细胞增多症,真性 骨髓纤维化 血小板增多 突变 基因 聚合酶链反应 JAK2V617F DNA突变检测 Myeloproliferative disorders Polycythemia vera Myelofibrosis Thrombocytosis Mutation Genes Polymerase chain reaction JAK2V617F Detection of the mutation 分类号 R551.3 [医药卫生—血液循环系统疾病]...