Per dot 10?l of sample was analyzed. Native filter retardation assay Frozen pancreatic tissues was homogenized in a 5-fold extra (w/v) of ice cold 50?mM Tris-HCl pH 7.5, 150?mM NaCl, 0.1% SDS, 0.5% Sodium deoxycholate, 1% Triton X-100, 0.25 U/l Benzonase and complete protease...
(b) Analysis of Notch1 and Jagged1 mRNA expression in response to IL-6 stimulation for 2 h and 4 h, as indicated. (c) Regulation of IL-6 mRNA expression by transfection of EGFP, Notch1 ICD or Notch1 ICDΔRAM in the RAW264.7 cells, as indicated. Values are significant at **P...