To measure growth curves, two biological replicates of each genotype were pre-cultured in 2 mL YPD medium in 14-mL sterile glass culture tubes with aluminum caps. Cultures were incubated for 24 h at 30 °C and 250 rpm in a shaking incubator (Thermo Scientific MaxQ 6000). After incubation,...
Effects of different pollination methods on bypassing pre-fertilization barriers in lily breeding To overcome incompatibility caused by pre-fertilization barriers in lily breeding,we used two species and three hybrids of lily,to study use of cut-style p... Q Chen,M Ding,M Yi,... - 《Journal...
So where is everybody? My family and I have an intense history with this part of the Southern California desert. The quietude of the dunes this time was in complete contrast to my first visit here eight years ago. On Thanksgiving weekend in 1987, I toured the East Mojave on a press tr...
water, and living organisms, involves the use of ecological floating beds (see Fig.5). The structure of the Plants Floating Bed is constructed using two types of thick bamboo tubes (TBT). The first type of TBT requires perforations at regular intervals for ...
(Merck, Germany) and 1.5% agar. Five seedlings were then transferred to Eppendorf tubes containing 200 μL medium with 10 μM fusion proteins or peptides. These tubes were then placed on a roller bank and cultured at 22°C under a 16 hr light/8 hr dark cycle. Ten μL of media was...
a上帝知道我做梦醒来之后的孤单 God knew I have a dream wake after lonelily[translate] aWork place to sleep Rests the work place[translate] aBaby Care & Health by Brand 婴孩关心&健康由Brand[translate] a国际商业模式 International business model[translate] ...
Fluorescence resonance energy transfer analysis of lily pollen tubes coexpressing CFP–LLP-Rop1 and YFP–LLP-12-2 demonstrated that LLP-12-2 is a target RIC protein of active LLP-Rop1, but the interaction between LLP-Rop1 and LLP-12-2 proteins is probably irrelevant of dehydration in the ...
[84]. The same function was reported in lily [85]. We observed high expression of theTEL1andFimbrin 2genes at stage E-L 15 in both clones, however as development progressed towards stage E-L 27 a significant repression of both genes in the mutant was evident in their very low RPM ...
(50mM Tris-HCl, pH 7.4) and 10 mM MgCl 2 using a PD- 10 desalting column(Amershan, Biosciences).The purified poly his- tagged luciferase was collected with 1.5 mL tubes and stored in portions of 0.5 mL at - 20 e .Unless stated otherwise, all steps of pur-ification were performed ...
the reaction mixture was extracted with 4 mL toluene and homogenized in a vortex. The chromophore containing the toluene was aspirated from the aqueous phase and the absorbance was read at 520 nm; immediately after, the terminated reaction in glass tubes placed in an ice bath, using toluene as...