图1 等温滴定量热法 (Isothermal Titration Calorimetry, ITC) 技术原理 案例一：MST 、Pull down、CD 技术在小分子与蛋白相互作用中的应用 2024年，苏州大学周芳芳研究团队在 Cell 杂志上发表了题为 “Alanyl-tRNA synthetase, AARS1, is a lactate sensor and lactyltransferase that lactylates p53 and ...
The novelty of this paper is to assume that the binding rate varies per titration step due to the change of the ligand concentration and to use this information in the Wiseman fitting. We do not claim to produce the most accurate values of the binding parameters, we rather present a novel...
The interactions of poly(n-butyl acrylate)-b-poly(acrylic acid) (PnBA-b-PAA) block copolymer nanoparticles with lysozyme were examined by a stopped-flow technique consisting of small-angle X-ray scattering (SAXS) and isothermal titration calorimetry (ITC). Nanoparticles composed of PnBA-b-PAA ...
Experiments of 15N chemical exchange saturation transfer and 15N R2 dispersion allowed the determination of Ca2+ dissociation rates (∼30 s−1). NMR titration curves of residues in the Ca2+-binding region were sigmoidal because of the contribution of chemical exchange to transverse magnetization ...
图1 等温滴定量热法 (Isothermal Titration Calorimetry, ITC) 技术原理 案例一:MST 、Pull down、CD 技术在小分子与蛋白相互作用中的应用 2024年,苏州大学周芳芳研究团队在 Cell 杂志上发表了题为 “Alanyl-tRNA synthetase, AARS1, is a lactate sensor and lactyltransferase that lactylates p53 and contributes...
Based on the fitting of SAXS curves, the number of micelles per volume increases with time, the core size remains nearly constant, and the thickness of the shell decreases. Such findings justify the model proposed in our previous papers.SergeyK.Filippov...