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Radiocarbon dating Radiocarbon determinations from Castelcivita, Cavallo, Cala and Oscurusciuto are reported in Tables1–4, respectively. Analytical data associated with the radiocarbon AMS dates from the first three sites is given in the Supplementary Information (Supplementary Data2–4). At Castelcivi...
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10 min PCR products were run by electrophoresis on 15% agarose gel, containing 05 mg/ml of SYBR green gel staining, purified (Sure Clean method, Bioline), and sequenced with the ABI Big Dye™ Terminator Cycle Sequencing version 20-ABI PRISM (Applied Biosystems, Foster City, USA) kit...
The rocks belonging to different complexes of the Austroalpine domain in the Western Alps, originally part of the thinned continental margin of the Adria plate, recorded heterogeneous metamorphic climax conditions and P–T–d–t paths during the Alpine evolution (e.g. Dal Piaz et al.1972; Compa...
(Illumina San Diego, CA, USA) using a 350-bp setting and following the manufacturer’s instructions. The HiSeq X Ten Reagent Kit v2.5 for 2 × 150 cycles and a HiSeq X Ten platform (Illumina San Diego, CA, USA) were then used to carry out sequencing experiments. The obtained...
(d) Fluid inclusions displacing the twinning lamellae in the matrix calcite, thus postdating at least some CH4 release to the calcite stability field. (e) Raman spectra of CH4-H2 fluid inclusions (see Supplementary Fig. 2 for Raman map of Graphite-bearing fluid inclusions). Abbreviations as ...