We found that IgG1, IgG3 and IgG4 were also elicited by group C polysaccharide using a solid phase radioimmunoassay and monoclonal, isotype-specific secondary antibodies. These data are best explained by the presence in the IgA-deficient individuals of multiple defects in T cell regulation of ...
Isotype controls allow you to distinguish between specific antibody staining and background staining in your experiments. Find out how to choose the best isotype control for your experiment.
Isotype controls are critical negative controls to identify non-specific binding caused by the constant Fc region of antibodies. Antibodies used as isotype controls share the same immunoglobulin class/subclass (IgG, IgM etc.) and conjugation as the experimental antibody and are derived from the same...
In total, 19 8-month-old Texel sheep were used to study the isotype-specific serum antibody responses against infective larvae and adult worms of Haemonchus contortus . Group 1 sheep ( n = 7) were infected with 20 000 L 3 larvae (Week 0), treated with ivermectin 6 weeks post-infection ...
Binding of specific antibodies was detected by isotype-specific enzyme-labeled secondary antibodies, followed by incubation with chromogenic or fluorogenic substrates. Quantification was achieved by coating plates with Bet v 1 or 4-hydroxy-3-nitrophenyl acetyl–conjugated bovine serum albumin and detection...
Unclassified [#IABV2_LABEL_PURPOSES#] [#IABV2_LABEL_FEATURES#] [#IABV2_LABEL_PARTNERS#] Homepage Isotype & Other Controls Particularly in procedures usingantibodies it’s very important to prevent any non-specific binding of primary and secondary antibodies by using non-specific isotype controls. ...
Anti-β2-GPI antibodies were measured in the sera of 70 patients by solid-phase enzyme immunoassay in γ-irradiated polystyrene plates coated with human purified β2-GPI. Thirty-three out of the 70 patients were classified as having APS: three of them had primary, and 30 had secondary APS ...
R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels. Our NorthernLights fluorescent secondary antibodies are bright and resistant...
(2) easily distinguishing the isotype and target (HLA class I or class II) of the antibodies; and (3) correlating the antibody with specific graft ... RM Mckenna,SK Takemoto,PI Terasaki - 《Transplantation》 被引量: 793发表: 2000年 The bone marrow of multiple myeloma patients contains B ...
Antibodies, HelminthImmunoglobulin IsotypesIn total, 19 8-month-old Texel sheep were used to study the isotype-specific serum antibody responses against infective larvae and adult worms of Haemonchus contortus . Group 1 sheep ( n = 7) were infected with 20 000 L 3 larvae (Week 0), treated ...