Ternary complexes of isopentenyl phosphate kinase from Thermococcus paralvinellae reveal molecular determinants of non-natural substrate specificitybiocatalysiscrystallographyx-rayhemiterpenesphosphotransferases (phosphate group acceptor)terpenesCRYSTAL-STRUCTURE
Orthologs of the archaeal isopentenyl phosphate kinase regulate terpenoid production in plants. Proc. Natl Acad. Sci. USA 112, 10050–10055 (2015). Article PubMed CAS Google Scholar VanNice, J. C. et al. Identification in Haloferax volcanii of phosphomevalonate decarboxylase and isopentenyl ...
147 The mevalonate kinase gene (MVK) encodes an ATP:mevalonate 5-phosphotransferase that is localized to the peroxisome. Mevalonic acid is synthesized from 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA), a reaction catalyzed by HMG-CoA reductase, and is converted to mevalonate-5-phosphate by mevalonate...
We found that monoterpene biosynthesis was positively correlated by the expression of five genes encoding 1-deoxy-D-xylulose-5-phosphate synthase (VvDXSs), one encoding 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (VvHDR), three hydroxy-3-methylglutaryl-CoA synthases (VvHMGSs) and one ...
Eukaryotes, fungi, and some gram-positive bacteria produce IPP through the mevalonate (MVA) pathway whereas gram-negative and some gram-positive bacteria utilize the non-mevalonate or 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway. Provided as the tris-ammonium salt Alternate Names: Isopentenyl...
Improving the catalytic activity of isopentenyl phosphate kinase through protein coevolution analysisProtein rational design has become more and more popular for protein engineering with the advantage of biological big-data. In this study, we described a method of rational design that is able to ...
Substrate Specificity of an Isopentenyl Phosphate Kinasedoi:10.1016/j.bpj.2018.11.402Shanteri SinghErin M. ScullElsevier BVBiophysical Journal
A recently identified kinase that resides in proximity to genes for other MVA pathway enzymes was found to phosphorylate proficiently isopentenyl phosphate (IP) to IPP, suggesting an alternative route for the MVA pathway in Archaea. This IP kinase represents a potentially powerful tool for ...
To be successful, each kinase in this pathway should be permissive of a broad range of substrates. For the first time, we have probed the promiscuity of the second enzyme in the ADH pathway, isopentenyl phosphate kinase from Thermoplasma acidophilum, towards a broad range of acceptor ...
volcanii of both the phosphomevalonate decarboxylase and isopentenyl phosphate kinase reactions that are required for an alternate mevalonate pathway in an archaeon. These results also represent, to the best of our knowledge, the first identification and characterization of an archaeal phosphomevalonate ...