gene editingnon-homologous end joiningoff-target effectsdominant negative mutationsEpidermolysis bullosa simplex (EBS) is a blistering skin disease聽caused by dominant-negative mutations in eitherKRT5orKRT14, resulting in impairment of keratin filament structure and epidermal fragility. Currently, nearly 200...
We describe Surface Oligopeptide knock-in for Rapid Target Selection (SORTS), a novel method to select mammalian cells with precise genome modifications that does not rely on cell cloning. SORTS is designed to disrupt the target gene with an expression cassette encoding an epitope tag embedded ...
We chose to target bacteria from the Firmicutes as rep- resentatives from this phylum constitute a significant component of the human gut microbial core14–16, although they are poorly represented by cultured isolates and relatively few of the existing isolates have been assessed for their ...
Regulatory and enzymatic functions were assigned to regions of two lux operons. With transcriptional fusions between the lacZ gene on transposon mini-Mu and the target gene, expression of lux operons could be measured in the absence of light production. The direction of transcription of lux ...
Before expression analysis, the same primers were used for sequencing of the target gene fragments including the miRNA binding site. Real-time qPCR experiments were carried out using 20 ng of cDNA and the iQ SYBR Green Supermix (Bio-Rad Laboratories, Richmond, CA) following the manufacturer’s...
Molecular analysis of an enhancin gene in the Lymantria dispar nuclear polyhedrosis virus. A Lymantria dispar nuclear polyhedrosis virus (LdMNPV) gene has been identified that encodes a homolog to the granulovirus (GV) enhancin proteins that are ... DS Bischoff,JM Slavicek - 《Journal of Virol...
Isolation and characterization of developmentally regulated novel target site from embryonic chick heart Differential gene expression is the primary determinant of numerous biological processes such as cell differentiation, proliferation, organogenesis, tumor ... S Neha,SK Goswami,R Vibha - 《African Journal...
1) were made to ensure the accessibility of SpyTag from the cytosol. Additionally, we chose to target mitochondria as they are essential for metabolism and energy production and by that an important subject for metabolomics and proteomics [29, 32, 55]. For mitochondrial targeting, the first 42...
Target labeling, however, demands a choice of strategies often unable to unify sensitivity and specificity. A less specific label such as a metabolic dye can be employed to detect the general presence of a certain group of particles, for example, bacteria in blood. Yet, with nonspecific ...
indicating gene flow between the WHG and Paleo-Siberian lineage. However, the tests were not significantly different from 0 for the Polish Mesolithic individuals, indicating genetic similarity to WHGs (Supplementary Fig.13a). To increase the power of the test, we calculated f4(chimp, Sidelkino; ...