Characterization of stem cells is based on the creation of embryoid bodies is a routine process used to classify human-induced pluripotent stem cells into separate lineages of cells. Embryoid bodies can be formed by suspension culture, hanging drop culture and semisolid media. Growth factors play ...
This report describes the isolation and in vitro expansion of human colon stem cells from normal tissues. Cells with high levels of the membrane receptor EPHB2 are shown to have characteristics of intestinal stem cells, and the authors optimize culture c
46. A method for differentiating a mesenchymal connective tissuederived stem cell in vivo comprising the steps of (a) obtaining tissue from a mammal; (b) establishing a population of adherent cells; (c) recovering said mesenchymal connective tissuederived stem cells; (d) culturing mesenchymal conne...
METHOD FOR ISOLATION OF STEM CELL 专利名称：METHOD FOR ISOLATION OF STEM CELL 发明人：YONEYAMA, Hiroyuki, c/o STELIC INST. OF REGENERATIVE MEDICINE 申请号：EP06843245.9 申请日：20061226 公开号：EP2022846A1 公开日：20090211 专利内容由知识产权出版社提供 专利附图：摘要：The present inventors ...
The skin is a rich source of readily accessible stem cells. The level of plasticity afforded by these cells is becoming increasingly important as the potential of stem cells in Cell Therapy and Regenerative Medicine continues to be explored. Several protocols described single type stem cell isolatio...
Background Neural induction of human pluripotent stem cells often yields heterogeneous cell populations that can hamper quantitative and comparative analyses. There is a need for improved differentiation and enrichment procedures that generate highly pure populations of neural stem cells (NSC), glia and ...
the magnetic-bound cells of interest are immobilized until removed from the magnet, while the supernatant is discarded. For negative selection, the cells of interest are in the supernatant. For cell depletion, cell-specific magnetic beads are used to remove specific unwanted cell types from a mixe...
including myogenesis. The discovery of the pivotal role played by PcG factors during myogenic differentiation relied on the possibility to culture myogenic cells in vitro. We describe here the methods currently used to isolate muscle stem cells (MuSCs) both from single myofibers and from bulk muscles...
Methods to accelerate the isolation of novel cell lines with limited potential for differentiation from pluripotent stem cells.West, Michael DSargent, GeoffreyMurai, James TKessler, StevenChapman, KarenLarocca, David
Background Neural induction of human pluripotent stem cells often yields heterogeneous cell populations that can hamper quantitative and comparative analyses. There is a need for improved differentiation and enrichment procedures that generate highly pure populations of neural stem cells (NSC), glia and ...