By combining the stringency of guanidinium-thiocyanate lysis with the speed and ease-of-use of silica-membrane purification, the ISOLATE II RNA Mini Kit provides a fast method for the purification of high-quality total RNA from animal and plant cells and tissues as well as cultured cells, bacte...
ASSOCIATED PRODUCTS PRODUCT ISOLATE II RNA Mini Kit ISOLATE II RNA Mini Kit ISOLATE II RNA Mini Kit ISOLATE II RNA Plant Kit ISOLATE II RNA Plant Kit RiboSafe RNase Inhibitor PACK SIZE 10 Preps 50 Preps 250 Preps 10 Preps 50 Preps 2,500 Units CAT NO. BIO-52071 BIO-52072 BIO-52073 BIO...
Bioline IS..特点:1) 从各种样本中提取高纯度总RNA而设计。试剂盒适用于提取动物组织、培养细胞、细菌组织、酵母、体液(无细胞)、石蜡包埋组织以及很难提取高质量RNA的生物样本(如:小鼠肝和脑、各种肿瘤细胞株、链
Whole genome expression analysis. Colon samples were homogenized in buffer RLT (Qiagen, California, USA) and total RNA was extracted using an AllPrep DNA/RNA/Protein Mini Kit (Qiagen) as per the manufacturer's protocol. The RNA quantity and purity was determined using a Nanodrop ND-1000 ...
Quantification of ZIKV RNA was determined by TaqMan one-step RT-qPCR on a Bio-Rad CFX96 Touch Real-Time System using an iTaQ Universal Probe One-Step Kit (Bio-Rad) under standard cycling conditions. The primer set used to detect ZIKV RNA (generated by Integrated DNA Technologies) can be ...
RNA was processed as described previously [160]. Briefly, RNA was extracted using RNeasy mini kit (Qiagen, Carlsbad CA), and samples obtained from three different cultures were pooled and amplified following the MessageAmp II Bacteria procedure (Ambion, Austin TX). 1 μg of RNA was poly...
Recruitment of PfSET2 by RNA polymerase II to variant antigen encoding loci contributes to antigenic variation in P. falciparum. PLoS Pathog. 2014;10:e1003854. Article PubMed Central PubMed Google Scholar Download references Acknowledgements This work was supported by the National Natural Science ...
A recent study showed that disrupting PfSET2/RNA pol II interactions in transgenic parasites induced rapid var gene expression switching [41]. The ability of the wild isolate to accelerate the var gene expression switching needs to be further studied. Frank et al. [19] indicated that despite ...
The ISOLATE II Plasmid Mini Kit provides a simple, efficient column-based method for the isolation of plasmid DNA from bacterial cultures, without the need for hazardous reagents such as phenol. By combining SDS/alkaline lysis with the speed and ease-of-use of silica-membrane purification, the ...
ISOLATE II RNA Plant Kit Product Manual Product Manual | www.bioline.com/isolate 2 RNA Plant Kit ISOLATE II RNA Plant Kit Table of Contents ISOLATE II RNA Plant Kit 1 Kit contents 2 Description 3 Storage 4 Safety information 5 Product specifications 6 Equipment and reagents to be supplied by...