Error bar, mean ± SD. (B) Strategy for MSTN targeting and SMG deletion. Depicted is i) the schematic outline of the pig MSTN gene, with exons shown in orange and the homology used in the donor DNA in purple (5′ homology arm) and blue (3′ homology arm); ii) schematic map ...
The Ucp1-/- rats were generated by CRISPR-Cas9 system and presented decreased BAT volume. 2-months old Sprague Daw- ley (SD) wild-type (WT) and Ucp1-/- rats were treated with ISO intraperitoneally 30 mg/kg once adanluyuanyindanbao...