Our findings will pave the way for more effective and targeted therapies via studies of drug targets at the isoform level.doi:10.1038/s41598-019-50224-xJun MaJenny WangLaleh Soltan GhoraieXin MenPenggao DaiScientific Reports
However, most researches were restricted in gene-level expression. mRNA isoforms, especially tumor-specific isoforms have not been fully explored in tumor. Here, by analyzing RNA-seq data derived from hundreds of samples in TCGA projects, we comprehensively characterized the expression variations of ...
We compared ORQAS with ORFquant38, another method to calculate translation at isoform level from Ribo-seq. We run ORFquant on the same samples analyzed with ORQAS. Looking at the annotated ORFs, ORQAS detected more genes with multiple ORFs consistently across all samples tested (Fig.1f, Suppl...
Deathofknockoutneuronsinculturecanberescuedbyexpressionof thebetaisoform. 神经细胞死亡的基因敲除的文化可以救出表达测试亚型。 www.syyxw.com 3. Indeterminingof ACTisoformlevelthecrossedimmunoelectrophoresismethodbyBog-Hansenwasused. 在确定青蒿素亚型水平交叉免疫电泳法沼泽,汉森用。
SQANTI Protein extends SQANTI3 transcript-centric classifications to the protein isoform level, considering how three key protein sequence elements—the N-terminus, the identified splice junctions, and the C-terminus—compare to reference protein isoforms (Fig. 3b). SQANTI Protein considers the full-...
Yang etal. BMCGenomics (2016) 17:631 DOI 10.1186/s12864-016-2852-6DATABASE Open AccessISOexpresso: a web-based platform forisoform-level expression analysis inhuman cancerIn Seok Yang 1 , Hyeonju Son 1,2 , Sora Kim 1,2 and Sangwoo Kim 1,2*AbstractBackground: Alternative splicing events ...
d. 位置与缺失/存在的比较接近FDA对PFAM 区域变异的ID-level结果,显示按变异基因总数排名的前 15 个域家族。 差异转录水平分析 转录和AltTEM分别通过控制总基因表达及可变异构体的相对水平来调节基因活性。这两个过程可以通过差异基因表达(DE)和差异异构体使用率(DIU)进行计算分析。DE 中的统计显著性经常通过潜在的...
single-cell-Isoform-Sequencing-Analysis-Tools: New and powerful tools brings single-cell RNA sequencing to the Isoform level and single molecule resolution. Resources Readme Activity Stars 24 stars Watchers 1 watching Forks 1 fork Report repository Releases No releases published Packages No...
(translated from alternatively spliced transcripts), not genes, are the actual function carriers. Now, high-throughput RNA-seq technology is providing unprecedented opportunities to unravel protein functions at theisoformlevel. In this article, we review recent progress in the high-resolution functional ...
Calculating the gene-level expression measurements can be slow for large experiments. The*exprfunctions return matrices unlessmeas = 'all', in which case some additional feature metadata is returned and the result is adata.frame. indexes Theindexesslot of a ballgown object connects the pieces of ...