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VirK is recognized as a periplasmic protein that is necessary for the efficient secretion of plasmid-encoded toxins in Escherichia coli [27]. In many pathogenic bacteria, TolQ, one of the most widely distributed proteins, is one of the envelope proteins that constitutes the Tol system, ...
we added 100 µL of STC buffer to the centrifuge tube to resuspend the cells, and the suspension was spread evenly on the regeneration medium and incubated at 20 °C. The process was performed in three parallel treatment groups for each concentration, along with a blank control group in wh...