Cancer stem-like cells were the crucial cell subset in cancer cells, with self-renewal ability, heterogeneity and high tumorigenicity, mediating malignant proliferation and immune escape of cancers [9,10,11]. Previous studies had shown that ATC cells were derived from oncogenic mutations in the gen...
An MVA-based vector expressing cell-free ISG15 increases IFN-I production and improves HIV-1-specific CD8 T cell immune responsesdoi:10.3389/fcimb.2023.1187193AIDST cellsINTERFERON receptorsIMMUNE responseVACCINIAHIVThe human immunodeficiency virus (HIV), responsible of the A...
Show abstract Transcriptome and proteomic analysis of mpox virus F3L-expressing cells 2024, Frontiers in Cellular and Infection Microbiology Show abstract The Structure and Function of the Bunyavirus Nucleoprotein 2023, Chinese Journal of Biochemistry and Molecular BiologyThe...
LFA-1 is expressed on T cells, B cells, macrophages, neutrophils, and NK cells, and its best-characterized ligands are intracellular adhesion molecules (ICAMs) (Abram and Lowell, 2009). The interaction of LFA-1 with ICAMs is critical for adhesion and recruitment of immune cells to antigen-...
We further studied whether ubiquitin was a substrate of ISGylation in cells over-expressing ISGylation components and ubiquitin. To avoid pos- sible interference from ubiquitylation of ISG15 (~17 kDa), we used C-terminally HA-tagged ubiquitin (Ub-HA, ~8 kDa) and UBB+ 1 (~10 ...
Translation levels of Sendai viral phosphate protein (P protein) were monitored in ISG54- www.cell-research.com | Cell Research npg Crystal Structure of ISG54 1334 expressing HEK293T cells. Western blotting showed that overexpression of the ISG54 mutants cannot effectively inhibit the production of...
To our knowledge, this is the first description of a differential antiviral activity of an ISG15 protein against two betanodavirus species, and the first evaluation of the cytokine-like activity of a fish ISG15 protein on non-immune cells. 展开▼ 机译:ISG15是一种抗病毒蛋白,其通过与病毒...
In addition, co-transfection of plasmids expressing ISG15, Ube1L, UbcH8, and Herc5 resulted in robust ISG15 conjugation in non-IFN-treated cells, while the active-site cysteine mutant of Herc5 or a mutant lacking the RCC1 repeat region did not support ISG15 conjugation. These results ...
we immunopurified FLAG-TOP1 from cells expressing the ISGylation machinery as in Fig.5dand monitored its ISGylation status by using ISG15 antibody. In the presence of ISG15 WT, we could observe a signal corresponding to ISGylated TOP1, which is missing in cells expressing the conjugation-...
U251 cells expressing FLAG-HDAC6 wild type (WT) and derivative mutants were stably selected by G418 treatment after infection. Doxycycline (Dox)-inducible ISG15 Conjugation System Construction of the Dox-inducible ISG15 conjugation system was as follows. 2A peptide linkage constructs between Ube1L ...