并用于不同类型临床样本的检测。方法常规提取13株结核分枝杆菌和14株非结核分枝杆菌临床分离株DNA,9例结核分枝杆菌阳性肺结核患者和4例其他肺部疾病患者痰DNA,12例结核分枝杆菌阳性肺结核患者和7例健康者血浆DNA:设计合成IS6110和1S1081扩增引物和检测探针,建立ddPCR检测体系。应用该体系检... ...
结核分枝杆菌IS6110和IS1081微滴数字PCR检测体系的建立和应用
设计合成IS6110和IS1081扩增引物和检测探针,建立dd PCR检测体系.应用该体系检测分枝杆菌,痰和血浆3种DNA样本中靶标拷贝数,进行统计学分析.结果建立了能同时检测IS6110和IS1081的dd PCR体系,该体系检测2个靶标的线性范围分别为0.5~8 733和0.2~2 893拷贝/μl反应体系.该体系具有较好的重复性(r>0.95),以非结核...
Limited variation of DNA fingerprints ( IS6110 and IS1081) in Korean strains of Mycobacterium tuberculosis. Tuber Lung Dis , 1995 ,76 :3242329.Huh Y J,Ahn D I,Kim SJ,et al.Limited Variation of DNA fingerprints(IS6110 and IS1081) in Korean strains of Mycobacterium tuberculosis. Tubercle ...
目的建立结核分枝杆菌IS6110和IS1081微滴数字PCR(droplet digital PCR,dd PCR)检测体系,并用于不同类型临床样本的检测。方法常规提取13株结核分枝杆菌和14株非结核分枝杆菌临床分离株DNA,9例结核分枝杆菌阳性肺结核患者和4例其他肺部疾病患者痰DNA,12例结核分枝杆菌阳性肺结核患者和7例健康者血浆DNA。设计合成IS6110和...
Mycobacterium tuberculosis phylogeny reconstruction based on combined numerical analysis with IS1081, IS6110, VNTR, and DR-based spoligotyping suggests the existence of two new phylogeographical clades. J Mol Evol. 2001; 53 (6):680–689. doi: 10.1007/s002390010255. [ Cross Ref ]...
Mycobacterium tuberculosis phylogeny recon- struction based on combined numerical analysis with IS1081, IS6110, VNTR, and DR-based spoligo- typing suggests the existence of two new phylogeographical clades. J Mol Evol. 2001; 53: 680-689. PMID: 11677628...