Different lines were used which were either p53 wild-type or carried various types of heterozygous or homozygous p53 mutations. The presence of mutations was demonstrated to correlate with a lack in transactivating activity of p53. While UV-light effectively produced apoptosis in cells of all lines...
Wild-type p53 expression and activity were suppressed in the presence of tetracycline, whereas withdrawal of tetracycline resulted in the induction of p53 expression, cell cycle checkpoint activation, and DNA damage-induced apoptosis. The regulated expression of wild-type p53 resulted in the recovery ...
inversions inMus, an inversion that induced prematureHoxd13expression in the tail bud at E10 (deer mouse E12) produced mice with ~2.5 fewer caudal vertebrae than the wild type79. Consistent with these studies, we found lower levels ofHoxd13in long-tailed forest mice compared with higher levels...
We next examined natural data by performing whole-genome sequencing of 26 wild-caught guppies from the source population (nfemales = 16, nmales = 10, average coverage ≥13×, final dataset = 1,021,495 SNPs). Using multiple lines of evidence, we found a large haplotype on LG...
Wild-type, heterozygous and homozygous offspring, born to heterozygous parents, were approximately at the 1:2:1 Mendelian ratio. Appearance and behavioral traits of Myo6-C442Y mice Myo6-C442Y mutant mice were Discussion Mouse models are useful tools to investigate the pathogenesis of various ...
Derivation rates of mESC from wild-type, heterozygous, and homozygous blastocysts were 33.3%, 21.43%, and 3.85%, respectively, indicating that Floped-/-blastocysts had significantly decreased derivation rates. Respective outgrowth appearing rate five days after blastocyst attachment were 83.3%, 85.7%,...
The four different SOST gene bodies (parental wild-type subclone [wt-SOST], mut1-SOST, mut2-SOST, and SDmut-SOST) were then subcloned into a mammalian expression vector in which the ubiquitous RSV LTR controlled expression of the insert (fig. 5A). Download: Download high-res image (...
Mitosis was identical in fibroblasts derived from cenpB wild-type, heterozygous and null animals. Our studies demonstrate that CENP-B is not required for the assembly of heterochromatin or the kinetochore, or for completion of mitosis. 展开
Complete loss of wild-type TP53 in a nontransformed human epithelial cell line is preceded by a phase during which a heterozygous TP53 mutant effectively o... (2000) Complete loss of wild-type TP53 in a nontransformed human epithelial cell line preceded by a phase during which a heterozygous...
To determine whether there is a requirement for LIFR signalling in prenatal testis development, we first confirmed the absence of LIFR protein in new-bornLifrtm1b(EUCOMM)Hmgumice. Wild-type (WT), heterozygous (HET) and homozygous (KO) mice were generated as described in the materials and me...