(i) The R677A mutation was generated by PCR amplification of V5-DLC1 using oligo 1016 (mouse Dlc1 nucleotides 2368–2411 (NCBI entry NM_015802.3) except for nucleotides (underlined) that create a R677A mutation and a silent mutation generating an EcoRI restriction site: (GTC GGG CTC TTC ...
Publisher: Vigil GamesDeveloper: Jim TegleitRussian edition: "Games Publishers PARTNER"Installation: Microsoft Windows 2003 / XP / VistaDescription: Another continuation of the famous adventure computer game Smackey, where the player plays the role of a brave adventurer who gets at his disposal a ...