Uracil DNA-glycosylase (UNG) is a DNA repair enzyme that removes the highly mutagenic uracil lesion from DNA using a base flipping mechanism. Although this enzyme has evolved to remove uracil from diverse sequence contexts, UNG excision efficiency depends on DNA sequence. To provide the molecular ...
Uracil-DNA Glycosylase of Thermoplasma acidophilumDirects Long-Patch Base Excision Repair, Which Is Promoted by Deoxynucleoside Triphosphates and ATP/ADP, ... T. Haugland et al., "Uracil-DNA glycosylase of Thermoplasma acidophilum directs long-patch base excision repair, which is promoted by deoxy...
The involvement of poly(ADP-ribose) polymerase-1 (PARP-1) in both pathways has been investigated by using PARP-1-deficient cell extracts to repair single abasic sites derived from uracil or 8-oxoguanine located in a double-stranded circular plasmid. For both lesions, PARP-1-deficient cell ...
The base is cytosine. It is found in both DNA and RNA. The given base is a pyrimidine as it contains only one ring. Based on the types of atoms... Learn more about this topic: Nucleic Acid | Function, Structure & Importance
a variety of innovative methods for plasmid construction have been developed. Some of the most notable examples include sequence homology based methods such as Sequence and Ligation Independent Cloning (SLIC)3, isothermal Gibson assembly4, uracil-excision based cloning5,6, and yeast homologous recombina...
To make the DNA -> RNA transcribing happen - RNA takes a DNA strands - the template strand that runs from 3' to 5' (prime) - and it is uses to form single stranded RNA with all T's replaced by U's. (Thymine replaced by Uracil). The RNA now runs 5' to 3' prime and is ide...
Transient reprogramming by the expression of OCT4, SOX2, KLF4 and MYC (OSKM) is a therapeutic strategy for tissue regeneration and rejuvenation, but little is known about its metabolic requirements. Here we show that OSKM reprogramming in mice causes a g
RNA shares a similar structure to DNA, except it contains only one strand, rather than two — so it looks like just one half of a ladder. In addition, while RNA has three of the four nitrogen bases in common with DNA, it uses a base called uracil rather than thymine to pair with ...
4-thiouracil Sigma-Aldrich 440736 DRB (5,6-dichloro-1-β-D-ribofuranosylbenzimidazole) Sigma-Aldrich D1916 MTSEA biotin-XX linker ((MTSEA Biotincapcap; 2-((6-((6-((biotinoyl)amino)hexanoyl)amino)hexanoyl)amino)ethylmethanethiosulfonate)) Biotium BT90066 Dsk2 beads Home-made; see Tufegdzic Vi...
J Sivaraman,P Iannuzzi,M Cygler,A Matte 摘要: Pseudouridine (5-β- d-ribofuranosyluracil, Ψ) is the most commonly found modified base in RNA. Conversion of uridine to Ψ is performed enzymatically in both prokaryotes and eukaryotes by pseudouridine synthases (EC 4.2.1.70). The Escherichia ...