— The UV photolysis of the aromatic amino acid, tryptophan (Trp), in the Ca 2+ -binding protein, cod paralbumin, type III, was studied using electron paramagnetic resonance (EPR) spectroscopy in the temperature range 4–80 K. For the Ca 2+ -bound protein, irradiation with UV light (...
Structure prediction of the C-terminal region of the B chain indicated a possible type I or type III turn for residues C-G-G-P-R (B22-26) preceding the tryptophan. Exchanging Pro(B25) for D-Pro within the turn caused a severe structural rearrangement at the C terminus and a 96% drop...
Tryptophan207 is involved in the GTP-dependent conformational switch in the alpha subunit of the G protein transducin: chymotryptic digestion patterns of t... 1993 . Tryptophan207 is involved in the GTP-dependent conformational switch in the β subunit of the G protein transducin: Chymotryptic ...
Novel stand-alone RAM domain protein-mediated catalytic control of anthranilate phosphoribosyltransferase in tryptophan biosynthesis in Thermus thermophilus Regulation of amino acid metabolism (RAM) domains are widely distributed among prokaryotes. In most cases, a RAM domain fuses with a DNA-binding domain...
H. evSeq: cost-effective amplicon sequencing of every variant in a protein library. ACS Synth. Biol. 11, 1313–1324 (2022). CAS PubMed Google Scholar Busch, F. et al. TrpB2 enzymes are O-phospho-l-serine dependent tryptophan synthases. Biochemistry 53, 6078–6083 (2014). CAS PubMed...
Groen b,Johannis A. Duine b,Robert Verpoorte a 摘要: Tryptophan decarboxylase (EC 4.1.1.28) from Catharanthus roseus was purified to homogeneity. The native enzyme has an M r of about 96000 as estimated from native PAGE. After SDS-PAGE, three protein bands were visible corresponding with M...
Cardiac contractility is regulated by dynamic phosphorylation of sarcomeric proteins by kinases such as cAMP-activated protein kinase A (PKA). Efficient phosphorylation requires that PKA be anchored close to its targets by A-kinase anchoring proteins (AKAPs). Cardiac Myosin Binding Protein-C (cMyBPC...
Mohapatra4, Felix Sahm5, Günter Küblbeck6, Toshikazu Nakamura7, Hiroshi Funakoshi8, Christiane A. Opitz2,4, Wolfgang Wick2,9, Ricarda Diem2,3 & Michael Platten1,2,10 The catabolism of tryptophan to immunosuppressive and neuroactive kynurenines is a key metabolic ...
(Type AN-2 and B) and solutions of L-tryptophan, L-asparagine, and L-glutamine (Wako Pure Chemical Industries Ltd., Chuo, Osaka, Japan) were used. Norvaline solution (0.2 mM) in the kit was used as an internal standard. The procedure requires a concentration step on a proprietary ...
6). This indicates that C-mannosylation confers considerable additional stability to the MTRAP protein fold. Fig. 3: Tryptophan C-mannosylation stabilizes MTRAP. a Western blot of supernatant recombinant MTRAP (rMTRAP) and SPATR (rSPATR) expressed in yeast cells with and without co-expression...