ProAlanase digestion showed an improved phosphorylation profiling in proline-rich single protein N3ICD, compared with trypsin, as well as allowed to increase total sequence coverage of the protein by combining peptides generated by both proteases. A similar increase in total protein sequence coverage...
trypsin isozymes is expressed in Paneth cells, that trypsin colocalizes with HD5 and that this protease can efficiently cleave HD5 propeptide to forms identical to those isolatedin vivo. By acting as a prodefensin convertase in human Paneth cells, trypsin is involved in the regulation of innate...
3b). LC-MS/MS analysis of trypsin-digested samples of these proteins confirmed that this mass shift was due to C-mannosylation on the two WxxW motifs within MTRAP (Supplementary Fig. 5), and quantitation by LC/MS enabled an estimation of the occupancy at each site (Fig. 3c, ...
The search was performed with trypsin as enzyme and iodoacetamide as cysteine blocking agent. Up to two missed tryptic cleavages and methionine oxidation as a variable modification were allowed for. Search tolerances were set to 10 ppm for the precursor mass and 0.05 Da for fragment masses, and...
Trypsin-like serine proteases are involved in a large number of processes including digestive degradation, regulation of developmental processes, yolk degradation and yolk degradome activation. Trypsin like peptidases considered to be involved in digesti
after which proteins were resuspended in 50 μL of 6 M urea and subsequently diluted to reduce urea concentration to <1 M. Proteins were digested with trypsin (final concentration 0.2 μg/μL) on an overhead rotor at 12 rpm overnight. Digested peptides were subsequently acidifie...
Martone CB, Busconi L, Folco EJ, Sanchez JJ (1991) Detection of a trypsin-like serine protease and its endogenous inhibitor in hake skeletal muscle. Arch Biochem Biophys 289:1–5 Article CAS PubMed Google Scholar Murphy G, Stanton H, Cowell S, Butler G, Knauper V, Atkinson S, Gavrilo...
The soluble protein fraction was isolated by centrifugation, reduced and denaturated in 100mM TEAB, 20mM TCEP, 0.05% (w/v) RapiGest at 55C for 20min, alkylated with 55mM CAA at RT for 30 min, digested with LysC (0.05mg of LysC/mg ...
Mesenchymal stem cells (MSCs) are an attractive source of adult stem cells for therapeutic application in clinical study. Genetic modification of MSCs with beneficial genes makes them more effective for therapeutic use. However, it is difficult to transd
Generally, animals extract nutrients from food by degradation using digestive enzymes. Trypsin and chymotrypsin, one of the major digestive enzymes in vertebrates, are pancreatic proenzymes secreted into the intestines. In this investigation, we report t