Briefly, DNA was isolated using the Genomic Maxi AX kit (A&A Biotechnology, cat. no. 995 − 10) and sequenced in a hybrid mode using the following sequencers: i) Illumina MiSeq (Illumina, San Diego, United States) - the library was constructed using the NEB Ultra II FS kit ...
Despite many associated features scattered around the genome, there were a few key genes and regions that seemed to have more mutational burden than others. In other words, these portions of the genome had the greatest variation relative to the most closely related Earth-bound strains. The genes...
There is clear evidence that the oceans are warming due to anthropogenic climate change, and the northeastern coast of USA contains some of the fastest war
BLAST1is widely used in molecular biology to search for nucleotide and protein sequences. Three decades after BLAST was introduced, there were major breakthroughs in structure prediction, and tools such as RoseTTAFold2and AlphaFold3emerged. Consequently, every protein sequence in the major sequence da...
“I worked in the lab for about 12 years. And for me there was a disconnect between what I was experiencing in the lab and what I was trying to do at home. In the lab, you’ve got single-use plastic waste, hazardous chemicals, energy-intensive instruments, all of that. It was not...
(Rodenburg et al., 2011). However, as was highlighted during the RAAIS workshops in both in Benin and in Tanzania, fertilizers are difficult to access in rural areas. In Benin, there is no well-developed private agro-dealer network and distribution infrastructure to support the supply of ...
There was a nonsignificant, negative relationship between the amount of viral and human RNA reads (R2 = 0.136). We then mapped and quantified on a transcript and gene model basis using STAR (v.2.7.5a) and Salmon (v.1.2.1)47,48. Reads mapping to multiple locations were dropped from ...
There are 4 main machine learning initiatives within the top 5 pharmaceutical and biotechnology companies ranging from mobile coaching solutions and telemedicine to drug discovery and acquisitions. 1. Mobile coaching solutions Mobile coaching solutions come in the form of advising patients and improving ...
Some may regard the use of biotechnology as a ‘technofix’ that fails to address the underlying causes of welfare problems in animal husbandry; others may consider this use of biotechnology a non-ideal solution which is acceptable only as long as no radical revision of farming practices is ...
Brevilin A (purity > 98%) was purchased from Chengdu Desite Biotechnology Co. Ltd. (Sichuan Provience, China). BCA protein assay reagents were purchased from Thermo Fisher Scientific (Thermor Fisher, MA, USA). 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and ...