contributes to only a fraction of the total H3 pool (3–17%)14. However, it has a dominant effect as it drastically reduces overall levels of the repressive H3K27me3 mark in cells14,15,16. In vitro, H3K
Sequences were obtained on the Illumina MiSeq platform in a 2 × 300 bp paired-end run aiming for >50,000 reads per sample (CeBiTec), following the standard instructions of the 16S metagenomic sequencing library preparation protocol (Illumina). The workflow and scripts used in this stu...
The RNA sequencing (RNA-seq) library preparation was done with 150 ng of input RNA using the Illumina TruSeq Stranded mRNA Library Prep Kit. Paired-end RNA-seq were performed with Illumina NextSeq sequencing instrument (Helixio, Clermont-Ferrand, France). RNA-seq read pairs were mapped to ...
Next-generation sequencing libraries were prepared from the purified mRNA using the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs). An equimolar pool was prepared and sequenced on a NextSeq 550 (Illumina) at the IRB. A minimum of 32.2 million reads were obtained for...
Illumina library preparation was done using the Kapa Hyper Prep Kit, as described previously (Novakovic et al., 2016). Genetic Analysis 200FG Cohort The 200FG cohort comprises healthy individuals of Western European descent which gave written informed consent to donate venous blood to use for ...
The m6A-enriched RNA was eluted from the beads, purified by using RNeasy Mini Kit (Qiagen), and then sent for sequencing with the Illumina PE150, following the protocol from company (Novogene). For methylated RNA immunoprecipitation (MeRIP)–quantitative polymerase chain reaction (qPCR), m6A-...
the Illumina DNA Prep workflow. DNA was denatured according to the protocol and diluted to a final loading concentration of 8 pM combined with a 5% PhiX spike-in (PhiX Control v3, Illumina) for sequencing on a v3-flowcell 2 × 300 bp on Illumina MiSeq (Illumina, San Diego, CA, USA)...
molecular weight and DNA concentrations. Pooled samples were purified using calibrated Agencourt AMPure XP beads and used to prepare the DNA libraries following the Illumina TruSeq DNA library preparation protocol. The sequencing was performed at the molecular research DNA laboratory (Shallowater, TX, ...
[23]. Each Vβ-specific primer and Cβ primer were modified to contain the universal binding sequence at 5′ end for Illumina library construction with indexing primers to sequence the Vβ–Dβ junction region, to permit good coverage of CDR3 with Illumina pair-end reads. Final PCR ...
Ribosome-depleted RNA preparations were used for double-stranded cDNA synthesis using the SuperScript II kit (Thermo Fisher Scientific, Waltham, MA, USA), and the samples were processed with the transposon-based chemistry library preparation kit (Nextera XT, Illumina, San Diego, CA, USA) followed...