Scans were recorded using an excitation wavelength of 275 nm and an emission wavelength ranging from 300 to 400 nm. Slit widths for both excitation and emission were kept at 4 nm, with a photomultiplier voltage of 750 V. References 1. Bochman, M. L., Paeschke, K. & Zakian, ...
The MS2 scans were performed in the ion trap with CID collision energy 30% and in the Orbitrap with HCD collision energy 40% back-to-back for each precursor. The raw data were processed on Proteome Discoverer 2.1 using the SequestHT search engine. The node for SequestHT included the ...
Survey scans of peptide precursors were collected from 350–1350 Th with an AGC target of 1,000,000 and a resolution of 240,000 in the orbitrap followed by Higher-energy collisional dissociation (HCD) tandem mass spectrometry (MS/MS) turbo scans taken in the ion trap. The resulting LC-MS...