after artificial transformation process without paadB, and after natural transformation with paadB. Whereas only theP.dispersa10FZSS14 isolate expressed theintI1gene without previous stresses, transcription was observed in some of the remaining environmental strains after transformation or exposure...
hESCs were transfected with siRNAs (negative control-12935300, NANOG-HSS188276 and OCT3/4-HSS143401, ThermoFisher Scientific) (100 nM) with RNAiMAX (ThermoFisher Scientific) according to the manufacturer’s protocol. Colony formation assay hESCs transduced with lentiviruses were dissociated into sing...
pseudomallei K96243 with an unmarked intragenic deletion in BPSS1498 (Δhcp1) This study E. coli S17-1 λpir (pMo130-ΔBPSS1498) Donor strain containing pMo130-Δbpss1498 plasmid; Smr Tpr Pbs Kmr 23 a Ipm, imipenem; Caz, ceftazidime; Chl, chloramphenicol; Cip, ciprofloxacin; Augmentin,...
cBioPortal (www.cbioportal.org) [34,35] is an online open website for analyzing and visualizing multidimensional cancer genomics data. We selected data from ovarian cancers in the “Query” section, and entered ST14 in “Query by Gene” section, used cBioPortal for further analysis. The type a...
Cells were harvested 3 days following transfection with StealthTM siRNA duplexes (ThermoFisher Scientific) specific for HTATSF1 (assay IDs: HSS120650, HSS120651), SF3B1 (assay IDs: HSS146413, HSS146415), or a negative control (12935200). Total RNA was isolated and DNase I-treated using the...
Obesity is a worldwide epidemic characterized by adipose tissue (AT) inflammation. AT is also a source of extracellular vesicles (EVs) that have recently been implicated in disorders related to metabolic syndrome. However, our understanding of mechanisti
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