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Lactic acid bacteria may selectively produce one specific stereoisomer of lactic acid (d or l) or a mixture of them in various proportions. Such an ability is determined by the presence of the enzyme lactate dehydrogenase, which possesses stereospecific NAD+-dependent activity (Hofvendahl & Hahn...
The LAR ratio is an independent risk factor associated with increased in-hospital mortality rates in patients with AHF. Keywords: acute heart failure; lactate dehydrogenase to albumin ratio; in-hospital mortality; MIMIC-III; data mining 1 Introduction Heart failure (HF) is a chronic and ...
D-lactate can be oxidized to pyruvate by D-lactate dehydrogenase (D-LDH). There are two types of D-LDHs: NAD-dependent D-LDHs and FAD-dependent D-LDHs23,24. The NAD-dependent D-LDHs were found in some bacteria and belong to the D-isomer specific 2-hydroxyacid dehydrogenase superfamily;...
Lactococcus lactis, the well-known Gram-positive lactic acid bacteria used in the food industry, have recently gained attention as a prospective therapeutic. L. lactis strains have been shown to be a promising host for heterologous protein expression [43,44,45] and for the production of native ...
It had an unusual putative polyadenylation signal, ATTAAA, reported previously (67, 68), located 20 bp upstream of the poly(A) tail (Fig. 2). The cDNA clone encoded a prepro-protein of 386 amino acid residues with a predicted size of 43,069 Da. The two sequences determined by direct...
The dried extract was dissolved in water and the concentra- tion of L-lactic acid was determined by an enzyme-based L-lactate assay kit (Cayman), and calibrated by the total volume of the tissues. The enzymatic reaction was not interfered with D-lactic acid, pyruvate, phosphoenolpyruvate, 2...
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it is unclear which amino acid of tnt activates water to initiate the nucleophilic attack on the nicotinamide-ribosyl bond. it is conceivable that the flexibility of tnt enables a structural rearrangement that might translocate a distal residue closer to the nicotinamide-ribosyl bond. further experimen...
What is the cation reaction with water, cation Ka, anion reaction with water, and anion Kb of sodium acetate? Conjugate Bases: In Acid-Base theory, a conjugate base is a base with the general form X− that can gain a proton to become an acid...