Sodium fluoride is also a chemical reagent used as a source of fluoride ions to create other fluoride compounds. This is due to the strong ability of the fluoride ion to attach to other ions and to form stable compounds. NaF also has several other industrial uses. The compound can be used...
We compared a combina- tion of sustained-release sodium fluoride, calcium citrate, and cholecalciferol (SR-NaF group) with cal- cium and cholecalciferol alone (control group).METHODS: Eighty-five ambulatory women aged 65 years or older with 1 or more nontraumatic verte- bral compression ...
sodium fluoride gracs sodium hexafluo phosp sodium hydrate sodium hydroxide burn sodium hydroxymethane sodium hyposulphite sodium iodate sodium ion-sensitive sodium ipodate bilopt sodium loading test sodium methide sodium sodium peanutamphoace sodium pentadecane su sodium petroleum sulf sodium phosphate mono...
Then, the supernatant was removed, cells were washed twice with ice-cold PBS and harvested by scraping directly into lysis buffer (100 mM NaCl, 50 mM HEPES-NaOH pH 7.9, 1 mM EDTA, 5% glycerol, 0.05% NP-40, 0.1% SDS, proteases and phosphatases inhibitors − 10 mM sodium fluoride (...
The cells were scraped in the lysis buffer (50 mM Tris-Cl, pH 7.5, 150 mM NaCl, 1 mM EDTA, 1 mM EGTA, 50 mM sodium fluoride, 1 mM sodium orthovanadate, 2% SDS and a protease inhibitor mixture (one tablet of Roche Diagnostics (Mannheim, Germany) protease inhibitor mixture/25 ml)),...
The cross-linked cell pellets were resuspended in 600 μl of lysis buffer [20.3 mM Na2HPO4, 3.5 mM KH2PO4, 6.8 mM KCl, 342.5 mM NaCl (pH 7.2), 0.1% SDS, 0.5% sodium deoxycholate, 0.5% NP-40, 10 mM sodium fluoride, and 0.25 mM sodium orthovanadate] and ...
Briefly, cell or tissue samples were collected and lysed, after that, the lysates were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), transferred to polyvinylidene fluoride (PVDF) membranes, and blocked with 5% skimmed milk dissolved in tris-buffered saline tween-...
All media were supplemented with 10% standard or charcoal-stripped fetal bovine serum (steroid-depleted serum), 100 IU/mL penicillin, 100 µg/mL streptomycin, 4 mM glutamine, and 1 mM sodium pyruvate. Cells were cultivated in a humidified incubator at 37 °C and in 5% CO2 atmosphere. ...
Equal amounts of protein samples were denatured by heating at 100 °C for 5 min and then subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis for separation. Subsequently, the separated proteins were transferred from the polyacrylamide gels onto polyvinylidene fluoride membranes (Bio-...
Total cell protein extracts were prepared by solubilizing in Igepal CA-630 (syn. Nonidet P-40, NP-40; I 3021, Sigma) lysis buffer containing 1% (v/v) NP-40, 50 mM HEPES pH 7.5, 10% (v/v) glycerol, 150 mM NaCl, 2 mM EDTA, 50 mM sodium fluoride (NaF), 10 mM sodium pyruvate...