Sodium Fluoride Is Not Harmless
Sodium fluoride is also a chemical reagent used as a source of fluoride ions to create other fluoride compounds. This is due to the strong ability of the fluoride ion to attach to other ions and to form stable compounds. NaF also has several other industrial uses. The compound can be used...
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Protein was extracted from cells or tissues by using RIPA buffer and quantified by the BCA method. Subsequently, protein was electrophoresed on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and then transferred to polyvinylidene fluoride membranes followed by incubation with 5% nonfat powdered...
(20 mM Tris, 0.2 M NaCl, 1 mM phenylmethylsulfonyl fluoride, 16 μg ml−1RNase, 0.5 mg ml−1lysozyme). Samples were then sonicated and centrifuged at 21,130 ×g. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis was used to verify that the protein was present...
the transfectants were washed with cold PBS and lysed at 4 °C in a buffer containing 20 mm Hepes (pH 7.5), 10 mm EGTA (pH 8), 2.5 mm MgCl2, 1 mm DTT, 1% Nonidet P-40, 40 mmβ-glycerophosphate, 2 mm sodium orthovanadate, 1 mm phenylmethylsulfonyl fluoride, 20 μg/ml aprotinin...
Long non-coding RNAs (lncRNAs) play vital roles in the pathogenesis of infectious diseases, but the role of lncRNAs in herpes simplex virus 1 (HSV-1) infection remains unknown. Using RNA sequencing analysis, we explored lncRNAs that were highly expressed
(phenylmethyl sulphonyl fluoride) and protease inhibitor cocktail tablet (Roche). Cleared supernatant was used for immunoprecipitation with anti-FLAG M2 beads. Bound complexes were washed extensively with TNET buffer and eluted with 3 × FLAG peptide in 20 mM NH4HCO3, pH 8.3. The eluate of ...
C in 1 ml of cell lysis buffer (50 mm HEPES, pH 7.5, 150 mm NaCl, 1 mm EDTA, 2.5 mm EGTA, 10% glycerol, 0.1% Tween 20, 1 mm dithiothreitol, 10 mmβ-glycerophosphate, 0.1 mm sodium orthovanadate, 10 μg/ml leupeptin, 2 μg/ml aprotinin, 100 μm phenylmethylsulfonyl fluoride)....