Video S1. ALEX smFRET derived trajectories of ALFA-tagged MPL coexpressed with JΑΚ2ΔTK-mEGFP in the presence or absence of 10 nM TPO variants (WT or TPO ), related to Figure 4 Video S2. Donor recovery upon acceptor bleaching of an MPL complex in the presence of TPO as used for ...
Generally, dietary fiber can be classified as soluble or insoluble fiber according to its solubility in water. Soluble fibers such as guar gum, psyllium, oligofructose, pectin, β-glucan, inulin, and glucomannan have shown notable effects in preventing obesity and obesity-related complications [[8]...
Briefly, the regular protocol is followed until the nuclear pellets are collected in buffer C, supplemented with protease inhibitor cocktail and sodium butyrate (1 mM). They are then softly dounce homogenized, and after centrifugation, the soluble nuclear fraction is quickly frozen and kept at À...
Many factors could modify the pace of this process, including diet, alcohol, and tobacco consumption, as well as contaminants present in water and food [5, 6]. Recently, the gut microbiota (GM) has also been correlated with CRC progression and found to be involved in modulating inflammatory ...
et al. Adipocyte-hepatocyte crosstalk in cellular models of obesity: role of soluble factors. Life Sci. 317, 121464 (2023). Article CAS PubMed Google Scholar Groeger, M. et al. Modeling and therapeutic targeting of inflammation-induced hepatic insulin resistance using human iPSC-derived ...
(right) One microgram of soluble MNase-treated chicken chromatin or 50-mer oligonucleotide duplex (200 nM) harbouring a nick with 30-P/50-OH termini was mock-treated (0) or treated with 1, 0.5 or 0.25 U T4 PNK to restore 30-OH/50-P termini. These DNA substrates were then incubated ...
Neurogenesis in the adult brain takes place in two neurogenic niches: the ventricular-subventricular zone (V-SVZ) and the subgranular zone. After differentiation, neural precursor cells (neuroblasts) have to move to an adequate position, a process known as neuronal migration. Some studies show that...
Physiology of the exocrine pancreas has been well studied in domestic and in laboratory animals as well as in humans. However, it remains quite unknown in wildlife mammals. Roe deer and cattle (including calf) belong to different families but have a common ancestor. This work aimed to evaluate...
Pentamidine is a water-soluble aromatic diamidine that has been in use for nearly 60 years. It is administered as either the isethionate form (Pentam 300) or methanesulfonate (Lomidine). The dose is 4 mg/kg/day for 10 days for adults and children. Pentamidine is effective against early-sta...
FGF13 may associate with MTs because colocalization was maintained in the growth cones after a treatment with a fixative containing 1% Triton X-100, which permeabilizes the plasma membrane and removes soluble proteins (Figure 1G). Moreover, FGF2 and FGF7, but not FGF13, were secreted into ...