Sodium diacetate is a free flowing acidic sodium widely used as food flavoring, preservative, and pH buffer. The benefits of using...
Nicotinic acid adenine dinucleotide phosphate (NAADP) is a potent Ca2+-mobilizing second messenger which uniquely mobilizes Ca2+ from acidic endolysosomal organelles. However, the molecular identity of the NAADP receptor remains unknown. Given the necess
5 and 7). Starting from Rad24 and counterclockwise, in the first four nucleotide binding pockets, the Walker A motif (P-loop, see sequence alignment) wraps the triphosphate, and the P-loop lysine forms a hydrogen bond with the phosphate group; the first acidic D/E of the Walker B motif...
TR-J and TR-E were unstable at above 50 and 40 °C, respectively, and were more stable at alkaline pH than at acidic pH. Thermal stabilities of TR-J and TR-E were highly calcium dependent. These purified trypsin enzymes were inhibited by serine protease inhibitors, such as TLCK and ...
As reported before, two endothermic peaks with a pre-transition at ~36 °C and a main denaturational transition at ~42 °C is observed for collagen in acidic solut 胶原的耐热性依赖性在12.4毫米钠醋酸盐缓冲(酸碱度4)对Al2 (SO4)的集中3由US-DSC学习了(。 1). 如报告前面,二个收热峰顶以前...
[H + ] and pH = pKa. Acidic Buffer Example: Acetic acid (HC2H3O2), sodium acetate (NaC2H3O2) NaC2H3O2 is a soluble salt that dissolves in water to give Na + and C2H3O2 - ions. Na + ions are neutral spectator ions, so can be ignored. The C2H3O2 - ions provide the conjugate...
Chitin deacetylase, active in the presence of acetate (96% of the enzymatic activity was retained in the presence of 100 mm sodium acetate), was purified t... K Tokuyasu,Mayumi OHNISHI-KAMEYAMA,K Hayashi - 《Journal of the Agricultural Chemical Society of Japan》 被引量: 307发表: 1996年 ...
We show that astrocytes, as judged by glial fibrillary acidic protein (GFAP) expression, never emerge from neuroepithelial cells on embryonic day (E) 11.5 even when STAT3 is activated, in contrast to E14.5 neuroepithelial cells. A CpG dinucleotide within a STAT3 binding element in the GFAP ...
1.25 μM (Supplementary Fig.1). This is similar to the inhibition of NH4Cl action potentials in isolated taste cells (see above). The higher affinity of the channel for Zn2+when activated at neutral versus acidic pH (IC50 = 0.20 mM, pH 5.5) is expected from its known pH ...
To extract GAGs, cells were homogenized in 500 µL of water containing protease inhibitors [protein estimation on a small portion of the samples was done at this stage], followed by adding an equal volume of 2× pronase digestion buffer (50 mM sodium acetate, pH-6.0). Samples were ...