Milliflex® PLUS Pump VHP hub Kit The Milliflex® PLUS Pump VHP hub kit is used for easy fluidic and electrical connection of the Milliflex PLUS Pumps at Sigma-Aldrich
(Sigma-Aldrich Cat# A5441), GAPDH (Sigma-Aldrich Cat# G9545), Bid (Cell Signaling Technology Cat# 2002), Bak (Cell Signaling Technology Cat# 3814), Bax (Cell Signaling Technology Cat# 5023), Mcl-1 (Cell Signaling Technology Cat# 5453), Bcl-2 (Santa Cruz Biotechnology Cat# sc-7382),...
Chemicals, cell culture media, supplements, culture flasks, plates, dishes and other plastic material were obtained from Sarstedt (Nümbrecht, Germany), Sigma-Aldrich (Deisenhofen, Germany) and Thermo Fisher Scientific (Waltham, USA), unless otherwise stated. Epothilone D (EpoD) was a kind gift...
Heparin Cofactor II, Human Plasma Heparin Cofactor II, Human Plasma, is a native human plasma protein that ihibits thrombin by forming a bimolecular complex in the presence of heparin. It shares homology with antithrombin III; Synonyms: Antithrombin BM a
The isopropanol was replaced gradually with paraffin (Paraplast Plus; Sigma Aldrich, St. Louis). Transverse root sections of 10-15 μm were made using a Reichert Jung 2050 SuperCut Motorized Microtome (Leica, Arnsberg, Germany). Ribbons were arranged on RNase-free, UV-treated, PEN-membrane 2.0...
Bacteria were embedded in agarose plugs and treated with lysostaphin (Sigma Aldrich, Munich, Germany), proteinase K (Merck, Darmstadt, Germany), and the restriction enzymeSmaI (Boehringer Mannheim, Mannheim, Germany) prior to electrophoresis. The electrophoresis program consisted of two blocks (10 ...
Acetic acid Merck Cat# 1.00063.1011 5-Fluoro-2′-deoxyuridine Sigma-Aldrich Cat# F0503 Neurobasal A Medium Gibco Cat# 10888-022 Neurobasal Medium Gibco Cat# 21103-049 Glutamax supplement Gibco Cat# 35050-061 B-27 plus supplement Gibco Cat# A35828-01 Tris-HCL Roth Cat# 90903 Cysteine Sigma...
control (35% formamide)63. All hybridizations were conducted for 2.5 h at 46 °C. Cells were imaged at ×1,000 magnification with an epifluorescence microscope (Axiophot II Imaging, Zeiss). Cells were counter-stained with 4′,6-diamidino-2-phenylindole (DAPI; Sigma-Aldrich) and for ...
(Ionicon Analytik, Austria). The production of O2was measured using the signal atm/z32 after the addition of 1 mg grape skin protein extract. Preliminary calibration using bovine catalase (Sigma-Aldrich) showed that maximum O2production (O2max) was proportional to the enzyme concentration in the...
Purified mouse IgG, IgG1 (Sigma-Aldrich), and IgM (Merck Millipore) antibodies starting at 12 µg/ml and seven consecutive 1:3 dilutions in PBS were used as standards. After PBST-washings, plates were incubated for 1 h with HRP-conjugated goat anti-mouse IgGs, anti-mouse IgM (...