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The protein concentration of all samples was measured using the BCA assay (Thermo Scientific; Rockford, IL). To minimize inter-sample variability for the evaluation of total protein staining as a loading control, we pooled CSF samples for our initial experiments. Eight pooled samples comprised of...
The eluates were applied to Protein A beads (Thermo Scientific, Rockford, IL) for IgG purification as per the manufacturer's recom- mendations, and the remaining non-IgG fraction is referred to as the IgA-enriched fraction. Aliquots were tested for purity of IgG frac- tionation by ELISA ...
Protein concentration of lysates were quantified with a BCA protein assay kit (Pierce Biotechnology, Rockford, IL, USA): 30 μg protein of each sample were run in duplicate on a 10% acrylamide SDS-PAGE gel using electrophoresis and transferred to a nitrocellulose membrane. For western blot ...
Protein concentration was measured using the BCA assay (Pierce, Rockford, IL, USA). 10–20 mg of sample protein was loaded per well of a 7.5% SDS precast gel (Bio- Rad, Hercules, CA, USA), and run for 30 min–1 h at 200 V. Kaleidoscope protein standards (Bio-Rad) were run ...
An aliquot of cell suspension was then stored at −80 °C using a proteinase inhibitor (Protease Inhibitor Cocktail kit, Thermo Scientific, Rockford, IL., USA), until use. IFN-γ production was assessed by a sandwich ELISA (Pig Interferon-γ; −IFN-γ, ELISA Kit, Cusabio, P.R. ...
This article will discuss the Illinois Health Care Surrogate Act, the term “decisional capacity” and advance directives including living will, power of attorney for healthcare, POLST and DNR.
The membranes were then incubated with the corresponding counter-antibody and the proteins revealed by enhanced chemiluminescence detection (SuperSignal West Femto System, Thermo Scientific, Rockford, IL, USA); alternatively, antigen/primary antibody complexes were detected with near infrared-fluorescence-...
Signals were visualized using enhanced chemiluminescence detection (Pierce, Rockford, IL). 2.8. Preparation of the HTLD-Medicated serum Adult BN rats were randomly divided into the control group and the HTLD group and treated with intragastric administration of PBS or HTLD-M (1 mL, twice a day)...
Protein concentration in the supernatant was calculated by using the micro bicinchoninic acid protein assay kit (Pierce, Rockford, IL). An aliquot of 50 μg total extract was mixed with protein loading buffer containing methoxyethanol, and boiled for 5 minutes before being loaded onto 15% SDS-...