Briefly, total RNA was homogenized with the QIAshredder mini kit (Qiagen), extracted with the RNeasy Mini Kit (Qiagen), and quantified by spectrophotometry. cDNA synthesis was performed using SuperScript III reverse transcriptase (Invitrogen). PCR reactions for ZNF827 and GAPDH were conducted with ...
By also transfecting in vitro-transcribed M3 plus-strand RNA containing silent mutations that render it resistant to the small interfering RNA, we were ... Michelle M. Arnold and Kenneth E. Murray and Max L. Nibert - 《Virology》 被引量: 81发表: 2008年 The mechanism of inhibition of reov...
siRNAs的是19日至21日2至3两个3'末端核苷酸出挑核苷酸的双链RNA。义链是相同的靶mRNA,而义链的mRNA的反义链是互补的。 翻译结果2复制译文编辑译文朗读译文返回顶部 siRNAs在两3'端是双倍陷入困境的有2到3核苷酸的伸出量的19到21核苷酸的RNA。感觉股与目标mRNA相同,而反义的股对感觉股和mRNA 补充。 翻译结果...
rosette leaves, buds, and flowers were collected, and isolated poly(A)+RNA were analyzed with LC-MS/MS. In all these tissues, thefiona1-1mutant plants had a significantly reduced extent (around 10~15%) of m6A modification in poly(A)+RNA compared with...
LC3/ATG8 has long been appreciated to play a central role in autophagy, by which a variety of cytoplasmic materials are delivered to lysosomes and eventually degraded. However, information on the molecular functions of LC3 in RNA biology is very limited.
one time in multiple samples, as well as discovery and analysis of different types of genomic features in a single sequencing run, from single nucleotide variants (SNVs), to copy number and structural variants, and even RNA fusions. N...
All occurred into only one strand, the lagging-strand template, a result that has strong statistical support (Figure 3 legend). In all cases, both plasmid and chromosome insertions occurred 3′ to a TTAC target, as expected. As these are distributed equally on both strands of the chromosome ...
The most striking feature of the Helicosporidium genome is the symmetry shown in strand bias of coding regions (Figure 1). The rRNA genes are nearly diametrically opposed, and all but two proteins and one tRNA on one side of them are on the same strand, while all but one tRNA on the ...
was measured by SYBR green fluorescence (SYBR Green master mix; Applied Biosystems). Raw data analysis was performed with the MxPro software (Agilent). All target gene transcripts were normalized to theEARtranscripts. All experiments were conducted at least three times with independent RNA isolation...
strand mature miRNAs, which recruit the Argonaute (AGO) protein to form the miRNA-induced silencing complex (miRISC).10miRISC binds target RNAs in correspondence of specific sequences termed miRNA Recognition Elements (MREs). The result is a decrease in target expression through RNA degradation or...