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RADNOR, Pa.,Jan. 29, 2025/PRNewswire/ -- Advanced Aesthetic Technologies, Inc. ("AAT") announced today that their partner inChinahas obtained approval to market their unique dermal filler Algeness®VL agarose facial injection f...
A 0.5-mL sample was mixed with 3 mL of 0.025 M sodium tetraborate (VWR, Radnor, PA) in sulfuric acid, and heated at 100°C for 10 min. After cooling, 0.1 mL of 0.125% carbazole (Sigma-Aldrich, St. Louis, MO) in absolute ethanol was added, and the samples were heated for another...
After three washing steps in PBS, the cells were blocked with 3% bovine serum albumin (BSA; VWR International, Radnor, PA, USA) in PBS containing 0.2% Triton X-100 (Merck-Sigma) for 1 h. Primary antibodies (Additional file 1: Table S1) were diluted in the same blocking solution and ...
All other reagents were purchased from VWR International (Radnor, PA) or Thermo Fisher Scientific (Hampton, NH) unless specified otherwise. Molecular cloning. With the exception of cloning Saccharomyces cerevisiae CPCT into pET vector (described below), CPCT refers to L. major CPCT in this ...
Ion-pairing high-performance liquid chromatography (IP-HPLC) was used to purify conjugated product with 0.1 M triethylammonium acetate (TEAA; Sigma-Aldrich, St Louis, MO, USA) as an ion-pairing agent on a C18 coated stationary phase and acetonitrile (ACN; VWR, Radnor, Pennsylvania, USA)...
Sections were counterstained with Hematoxylin-Mayer solution (Klinipath, Breda, The Netherlands), washed with tap water, dehydrated in alcohol and xylene and coverslipped in Pertex (VWR International, Radnor, PA, USA). IL-8 and CXCR1/2 antibodies cross-react only with human specimens, as ...
(VWR International, Radnor, PA, USA). The column was Ultra Aqueous C-18, 4.6 × 250 mm, with 5 μm particle size (Agilent, Santa Clara, CA, USA). The mobile phase consisted of 0.018 N H2SO4, pH 2.2, and a combination of flow rates ranging from 0.2 to 1.0 mL/min was used. ...
Yeast cultures were grown in either YPD (1% yeast extract) US Biological Life Sciences, Swampscott, MA, USA, 2% glucose (VWR, Radnor, PA, USA), 2% peptone (Thermo Fisher Scientific, Waltham, MA, USA) or in SD (0.67% yeast nitrogen base without amino acids and carbohydrates (US Biologica...
(Sigma-Aldrich Ltd., Dublin, Ireland) for 15 min at 37 °C, then washed with 0.1 M PBS (pH 7.2) again. Finally, the cells were transferred into a 96-well fluorescence microplate and measured by a microplate reader (SpectraMax M2e, Molecular Devices, Radnor, PA, USA) at the excitation...