RACE: rapid amplification of cDNA ends The origins of the anisotropic physical properties of CePtAs and LaPtAs were investigated by the optical conductivity and the XPS measurements. Optical conductivity spectra indicate that the property parallel to the -axis is semiconducti... MH Frohman - 《Pcr...
And the more we look, the more we will see that social factors(因素) like gender, race, and class do not determine the appearance of genius. As a writer says, future geniuses come from those with “intelligence, creativity, perseverance(毅力), and simple good fortune, who are able to ...
flooding actually makes the phobia worse. There is no way to know if a patient will respond well to flooding therapy, so the general consensus is that slow and steady wins the race.
The full-length coding sequence ofP.sinensis Dmrt1was obtained by RT-PCR and 5′ and 3′ RACE. The complete cDNA sequence ofP.sinensis Dmrt1was 2,409 base pairs (bp) (accession number KY964413), with a 230 bp 5′ untranslated region (UTR), a 1072 bp 3′ UTR, and an open rea...
Fresh off of his unexpected departure from President Donald Trump's Department of Government Efficiency, Vivek Ramaswamy has set his sights on becoming governor of Ohio, his home state Associated PressJan. 26, 2025 Freighter on the Move After It Was Freed From Ice on Frozen Lake Erie ...
Identities of algal and fungal partners were confirmed by DNA barcoding. Algal activity was examined using gas exchange and chlorophyll fluorescence techniques. Carbon transfer from algae to fungi was detected as 13C, assimilated by algae, transferred to the fungal polyol. Nine fungal partners ...
Both transcript types were produced by a confirmed carrier cow, as detected by 5' RACE. Full size image SLC4A2 transcript analysis To test the hypothesis that the biological consequence of the deletion mutation is the formation of a transcript lacking exons 2 and 3, we performed 5' RACE to...
The protein concentration was determined by NanoDrop 2000 (Thermo Scientific) and aliquoted and stored at −80 °C until further use. To study N1-PrP without an MBP tag, N1 was cloned into a pGATEV vector and expressed in and purified from E. coli BL21-DE3. Sample preparation Protein ...
DNA samples generated by ChIP from HPC7 or FMH9 chromatin were analyzed by Q-PCR using primers covering the defined regions of nuclease hypersensitivity and relative enrichments were determined by comparison with matched IgG ChIP controls. HoxA9, Meis1 and Pbx1 were found associated to the c-...
Leigh Van Valen showed that the probability of extinction of a population was constant over time and suggested an evolutionary arms race where survival is dependent on a population’s ability to adapt to changing selective pressures95. During times of intense selective pressure, selection for rapid ...