as well as an ORF encoding the outer membrane protein OmpW (K07275) (Fig.5c), which has been reported to play a role in biofilm formation44,45. As examples, PotD is a spermidine/putrescine-binding periplasmic protein, and its overexpression strongly promotes biofilm formation ...
A further strategy to reduce intracellular O2concentration in hydrothermal plumeUS. plumais the loss of superoxide dismutases (Table1), an enzyme that catalyses the transformation of superoxide (O2−) into molecular oxygen and hydrogen peroxide54. This is the primary antioxidant system in aerobic ...
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Because milk is a great source of protein, it helps build and repair muscle tissue. Plus, milk is naturally rich in calcium which is vital to building and maintaining bone strength throughout life. These benefits make milk a great choice for many populations, including growing adolescents, ...
Although respiration is more energy efficient, some cells favor glycolysis even when oxygen is available (aerobic glycolysis, Warburg effect). A leading explanation is that glycolysis is more efficient in terms of ATP production per unit mass of protein (that is, faster). Through quantitative flux...
After denaturation for 5 min at 95 °C, proteins were resolved in SDS-PAGE gels, transferred via wet transfer onto a nitrocellulose membrane (GE Healthcare, cat. no. GE10600001), and incubated overnight with primary antibodies in dilution 1:1000 at 4 °C. Antibodies used in this study: ...
Question: Is it safe to choose plant milk as a source of protein because of allergies or lactose intolerance? Ji Ying: Some people choose alternative milk because of milk allergy or lactose intolerance. Drinking milk c...
Aliquots of 30 μl were loaded on an 11% SDS-PAGE (100 V) and blotting to a PVDF membrane. For detection the primary antibody raised in rabbits against a FlaB peptide (Eurogentech) and an anti rabbit IgG–alkaline phosphatase coupled antibody (Sigma Aldrich, St Louis, USA) were used. ...
Primary NBs survival data mRNA gene expression analyses were performed within R2: a genomics analysis and visualization platform (http://r2.amc.nl), using datasets: NB Kocak (n = 649, GEO: GSE45547, patient cohort 1), NB Versteeg (n = 88, GEO: GSE16476 88/122, patient cohort...
is refractory to reprogramming in our mouse model3; however, we did observe a significant depletion of vitamin B12from the proximal tubules during reprogramming (Extended Data Fig.3b). The kidney is the primary site of B12concentration and storage in rodents, from where it is released for use ...