(57) cDNA encoding [summary] mammal, the AMP protein kinase, recombinant protein with an antibody against the polypeptide as well as their corresponding. Their use, including the characterization of proteins and other test of gene expression.ラジンダークマール...
The ATR-CHK1 DNA damage response pathway becomes activated by the exposure of RPA-coated single-stranded DNA (ssDNA) that forms as an intermediate during DNA damage and repair, and as a part of the replication stress response. Here, we identify ZNF827 as a component of the ATR-CHK1 kinase...
ZRANB3is a protein-coding gene with nucleic acid binding and endonuclease activity. TheZRANB3transcript is the target of nonsense-mediated decay (NMD) and is expressed in tissues relevant to T2D, including adipose tissue, skeletal muscle, pancreas, and liver (Supplementary Fig.3). We identified ...
Lactococcus lactis, the well-known Gram-positive lactic acid bacteria used in the food industry, have recently gained attention as a prospective therapeutic. L. lactis strains have been shown to be a promising host for heterologous protein expression [43,44,45] and for the production of native ...
The findings reported here will advance our knowledge of the nucleic acid metabolic processes in the malaria parasite. Access through your organization Check access to the full text by signing in through your organization. Access through your organization ...
MudPIT Analysis of Protein Complexes Halo purified proteins were precipitated with trichloroacetic acid, the resulting pellet was washed twice with acetone, and proteins were resuspended in buffer containing 100 mm Tris·HCl pH 8.5 and 8 m urea(13). Samples were incubated with 0.5 mm tris(2-carbo...
a Chinese scientist who is one of the pioneers of CRISPR research, founded Sherlock BioSciences in 2017. Using the improvement of the natural RNase activity of Cas13 protein, he established the SHERLOCKTM (Specific High Sensitivity Enzymatic Reporter unlocking) platform for rapid nucleic acid detectio...
The smaller ORF1 of segment 8 encodes the matrix protein, while the larger ORF2 encodes an RNA-binding structural protein also with interferon antagonistic properties [4, 9–11]. Regarding the genes coding for surface proteins, he genes exhibit a length polymorphism close to the 3' end called...
6)31,45. According to the tail displacement model, interactions of DNA-binding domains (DBD) of a nucleosome-binding protein with the nucleosome can displace histone tails from their DNA preferred binding modes. It makes tails more accessible for recognition by reader domains (Fig. 6). The ...
Here, we used 2CLCs to perform a small-molecule screen to identify new pathways regulating the 2-cell-stage program. We identified retinoids as robust inducers of 2CLCs and the retinoic acid (RA)-signaling pathway as a key component of the regulatory circuitry of totipotent cells in embryos....