The primer is the first layer of the paint system, which is used to improve the adhesion of the finish, increase the fullness of the finish, provide alkali resistance, provide anti-corrosion functions, etc., and ensure the uniform absorption of the finish, so that the paint system can play...
The primers used for Polo RNAi sequence amplification (600 bp) were forward: 5′-GAGGCGCTTCGTCTACGGAG-3′ and reverse: 5′-GATCGGGGATTTCGGGTTGGC-3′. Bioluminescence resonance energy transfer The KD–IDL (amino acids 1–349) portion of Polo N-terminally fused to RLucII (Luc-KD) and ...
Primers used for the qRT-PCR analysis are detailed in Supplementary Table 1. IAA and IAA-conjugate measurements Arabidopsis thaliana seedlings were grown in 21WL (constant LED light) for 3 days and treated for 3 h in the indicated conditions to measure auxin and its conjugates. Seedlings ...
Using aprimercan truly make all the difference. But those new to the world of makeup may wonder what, exactly, is the point. Makeup primers are used underneath eyeshadow, foundation, lipstick, mascara, and even nail polish to create a smooth base that helps keep your other products in p...
ambiguous bases were retained. If minor modifications affecting at maximum 2 bp of the V3−V4 primers used here were employed in the previous studies, the resulting amplicons were trimmed to matching start and end positions, assuming that any primer bias regardingSulfurimonaswould be negligible....
In addition to general product development processes, specific methodologies are often used in the development of software. These methodologies provide a framework for workflow, including how teams and different functions work together, as well as how and when specific steps are taken. Software developm...
Table S3. Primers used in this paper for cloning and mutagenesis. Figure S1. The sequencing results of LC3B-a, GABARAP-a and GABARAPL1-a. Figure S2. Primer specificity test for LC3B and LC3B-a transcripts. Figure S3. Alignment of human Atg8 family proteins. Figure S4. The interaction ...
When primers amplifying both ChoRE and PPRE were used (Figure 5C), a significant enrichment in PPARα binding within the proximal region of the Fgf21 promoter in liver of Pparα hep+/+ mice was observed (Figure 5D). While PPARα binding was elevated under fasting conditions, a significant ...
(n = 4, 4, and 3, respectively).fConstruct used to generate Tg(CaMKII-hA2AR) rats; Tg(CaMKII-hA2AR) animals present an overexpression of total A2AR in the hippocampus evaluated by in situ hybridization (both with the hA2AR probe and through cross-hybridization of the rat A2AR ...
Positive clones after transformations were screened by PCR and BamHI restriction digestion, finally sequenced (list of primers used are provided in Supplementary Table 1). pTRIP-CMV-GFP-FLAG-cGAS (Plasmid #86675)28, Nesprin tension sensor (pcDNA nesprin TS; plasmid #68127), and nesprin ...