Phenol red (PR) is used as a pH indicator in cell culture medium. We found that cell culture medium containing PR relaxed canine lingual arteries (LA) contracted by the thromboxane A2/prostaglandin endoperoxide (TPE) receptor agonist (15S)-hydroxy-11-alpha-9-alpha- (epoxymethano)prosta-5Z,...
Phenol red (PR) is the standard pH indicator in various cell and tissue culture media, as it provides a quick check for the health of the culture. PR has also been used in multiple protocols to detect cellular hydrogen peroxide as well as peroxidase activity from human peroxidase enzymes. Th...
Indicators are often used in titration reactions to signal that the equivalence point pH is reached. The indicators added are weak acids or bases that exhibit different colors based on the pH. An example of an indicator is Phenolphthalein.
Fast-replicating neurotropic herpesviruses exemplified by herpes simplex virus-1 (HSV-1) naturally infect the central nervous system (CNS). However, most individuals intrinsically suppress the virus during a primary infection and preclude it from signifi
5C). To further assess cell cycle dysregulation, we employed fluorescence ubiquitination cell cycle indicator (FUCCI) HT1080 cells. Live cell imaging of FUCCI cells identified a striking increase in G1 duration (red cells) following ZNF827 depletion (Fig. 5D), while no impact on S/G2-M ...
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An acid base indicator hasKHIn=3.0×10−5. The acid from of the indicator is red and the basic form is blue the change in(H+)required to change the indicator from 75% red to 75%blue is :- View Solution At what pH an indicator withpkb=4changes colour: ...
These results support that the hypermethylation of within-peak CpGs is an important indicator of super-enhancer deactivation during tumorigenesis (Skvortsova et al., 2019). Finally, we found ∼50,000 eRNA loci (or ∼62,000 associations) whose expression levels were associated with clinical ...
Conversion rate (Vmax) ) and affinity (Km) for 3-oxo-hexanoyl-, 3-oxo-octanoyl-, 3-oxo-decanoyl-, and 3-oxo-dodecanoyl- homoserine lactones, all from Merck Life Science S.L.U. (Madrid, SPAIN), was calculated using a pH indicator assay in 96-well plates, at 30 °C and pH 8.0 ...
Cells were then incubated with 1 µM crisp-17 in DMEM (without phenol red indicator) for 20 min and imaged at 37 °C under a humidified 5% CO2 atmosphere using a Zeiss LSM 880 two photon laser (excitation at 880 nm and emission simultaneously at 479–536 nm (ch.1) and ...