Phenol red (PR) is the standard pH indicator in various cell and tissue culture media, as it provides a quick check for the health of the culture. PR has also been used in multiple protocols to detect cellular hydrogen peroxide as well as peroxidase activity from human peroxidase enzymes. ...
Cells were then incubated with 1 µM crisp-17 in DMEM (without phenol red indicator) for 20 min and imaged at 37 °C under a humidified 5% CO2 atmosphere using a Zeiss LSM 880 two photon laser (excitation at 880 nm and emission simultaneously at 479–536 nm (ch.1) and ...
We questioned here whether GSK3β might have a role in the Ca2+ transfer from SR/ER to mitochondria at reperfusion. We showed that a fraction of GSK3β protein is localized to the SR/ER and mitochondria-associated ER membranes (MAMs) in the heart, and that GSK3β specifically interacted ...
Based on your experimental results, whyy is it possible to identify Phenol Red but not Phenolphthalein in an unknown that contains both? Why did the solution turn to pink at the end of the titration ? Describe clearly why a single indicator solution is only useful for measuring pH over a ...
By using CM-H2DCFDA, an intracellular ROS indicator, we found that silencing of APE1 significantly increased the ROS production in OE33 cells and promoted ROS generation in response to ABS treatment (P < 0.05) (Fig. 1 A and B). Similar results were obtained in CPB cells (P < 0.001) ...
pH is a measure of how acidic or basic a substance is from 0-14. 7 is a neutral pH. The closer to 14, the more basic a compound is. The closer to 0, the more acidic.Answer and Explanation: Become a member and unlock all Study Answers Start today. Try it now Create an account...
The cells were grown at 37 °C in a humidified incubator with 5% CO2 atmosphere. All experiments were performed in the medium containing Neurobasal without phenol red and 2% B27 without antioxidants (Gibco). For confocal analyses and cytotoxicity assays, 7–9 days-old primary cultures of ...
Brain-wide c-fos expres- sion is enhanced upon waking and after stimulation (Cirelli and Tononi 2000) and is a widely-used indicator of neu- ronal activity, including in zebrafish (Baraban et al. 2005; Reichert et al. 2019). In control experiments, caffeine-treated larvae showed on ...
The extent of urease production, which is observed due to the incorporation of the phenol red pH indicator, is proportional to the diameter of the pink halo surrounding the inoculum. involved in fatty acid β -oxidation and peroxisomal organisation was similarly down-regulated in all mutants (...
Conversion rate (Vmax) ) and affinity (Km) for 3-oxo-hexanoyl-, 3-oxo-octanoyl-, 3-oxo-decanoyl-, and 3-oxo-dodecanoyl- homoserine lactones, all from Merck Life Science S.L.U. (Madrid, SPAIN), was calculated using a pH indicator assay in 96-well plates, at 30 °C and pH 8.0 ...