ConclusionA lavage step including alcohol and chlorhexidine is more effective than alcohol alone for removal of excess phenol. The addition of chlorhexidine also contributed to inhibition of the action of phenol. This approach provides a suitable and effective means of diluting and removing any excess...
For RNA analysis, samples of the reactions were quenched with EDTA, supplemented with tRNA, extracted with phenol/chloroform/isoamyl alcohol (1:1:0.04), precipitated with ethanol, and electrophoresed on 12% polyacrylamide sequencing gels. Gels were visualized by phosphorimager and the data analyzed ...
P1 Phenol 95% (1 mM) Kirkwood et al. (2006) Chlorella sorokiniana 211/8k Ralstonia basilensis Sodium salicylate 1 mmol l−1 day (5 mM)a Guieysse et al. (2002) C. sorokiniana Pseudomonas migulae Phenanthrene 24.2 g m−3 h−1 (200–500 mg l−1) Muñoz et al. (2003a)...
An antioxidant may be used in the instant invention such as a substituted, sterically hindered phenol, such as a substituted ester of hydroxyhydrocinnamic acid. In one embodiment, the antioxidant element is a 3,5-dialkyl ester of hydroxyhydrocinnamic acid, and another embodiment is octadecyl 3,5...
(GC17761) is a 65-year-old male of Sudanese descent born to consanguineous (2ndcousin) parents (Fig.5c–e). He has two similarly affected sisters (F1:2, and F1:3). He also has an independent, maternally inherited, polycystic kidney disease (PKD) that does not segregate with the ...
Following organic extraction (25:24:1 (v/ v) phenol/chloroform/isoamyalcohol), the enriched mRNA was precipitated overnight with 2.5 volumes of an mixture of ethanol and 0.1 M sodium acetate (pH 6.5). Depletion of 16S and 23S rRNA was confirmed by 2100 Bioanalzyer (Agilent) and gel ...
The precipitated chromatin DNA was then purified by phenol–chloroform-isoamyl alcohol extraction and recovered by ethanol precipitation. ChIP-qPCR was performed, and results were calculated as a percentage of input DNA. Primer sequences for ChIP-qPCR are listed in Additional file 4: Table S1...
(10 mM Tris-Cl pH8.0, 25 mM EDTA, 100 mM NaCl,0.5% SDS) was added to the media, and incubate at 55 °C for 4 h with rotation. An equal volume of Phenol: Chloroform: Isoamyl Alcohol (Thermo Fisher) was mixed with the media and centrifuged with a swing rotor at 2000...
After DNAse I treatment for 1 h at 37°C, followed by phenol–chloroform–isoamyl alcohol extraction, RNA was precipitated with sodium acetate/ethanol and dissolved in DEPC-treated water. cDNA was synthesized at 42°C by superscript II (Invitrogen) using 0.5–1 μg RNA as template. The cDNA...
Phenol/Chloroform/Isoamyl Alcohol Thermo Fisher Scientific BP1752I400 NP-40 (Igepal CA-630) Sigma I8896 MboI New England Biolabs R0147 Benzonase® Nuclease HC Millipore 71205 ANTI-FLAG® M2 Affinity Gel Sigma-Aldrich A2220 3X FLAG® Peptide Sigma-Aldrich F4799 Trichostatin A Sigma-Aldrich T...